Evidence map›Paper›PMID 31940771›Full record

ArticleMicroorganisms2020

Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia.

Andrea Vergara, Hervé Boutal, Adrián Ceccato, Míriam López, Adrià Cruells, Leticia Bueno-Freire, Javier Moreno-Morales, Jorge Puig de la Bellacasa, Pedro Castro, Antoni Torres and 3 more

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.3field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 31 citations in OpenAlex.

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  7. Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024
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  16. Rapid Detection to Differentiate HypervirulentFrontiers in microbiology · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Andrea VergaraDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.ORCID 0000-0002-5046-4490
Hervé BoutalService de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d'Etudes et de Recherches en Immunonalyse, Université Paris-Saclay, F-91191 Gif-sur-Yvette, France.
Adrián CeccatoPulmonary and Critical Care Unit, Respiratory Institute, Hospital Clinic, 08036 Barcelona, Spain.
Míriam LópezDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.
Adrià CruellsDepartment of Medicine, School of Medicine, University of Barcelona, 08036 Barcelona, Spain.
Leticia Bueno-FreirePulmonary and Critical Care Unit, Respiratory Institute, Hospital Clinic, 08036 Barcelona, Spain.
Javier Moreno-MoralesISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain.ORCID 0000-0002-5251-8579
Jorge Puig de la BellacasaDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.
Pedro CastroDepartment of Medicine, School of Medicine, University of Barcelona, 08036 Barcelona, Spain.ORCID 0000-0002-6118-8970
Antoni TorresPulmonary and Critical Care Unit, Respiratory Institute, Hospital Clinic, 08036 Barcelona, Spain.ORCID 0000-0002-8643-2167
Francesc MarcoDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.ORCID 0000-0002-1863-6379
Climent Casals-PascualDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.
Jordi VilaDepartment of Clinical Microbiology, CDB, Hospital Clínic of Barcelona, University of Barcelona, 08036 Barcelona, Spain.ORCID 0000-0002-8025-3926
Hospital Clínic de Barcelona · ESBarcelona Institute for Global Health · ESCommissariat à l'Énergie Atomique et aux Énergies Alternatives · FRUniversitat de Barcelona · ES

Funding

European Regional Development Fund "A way to achieve Europe"Fundació Catalunya-La Pedrera PEP:HB-16-JF-VG-CInstituto de Salud Carlos III REIPI RD16/0016/0010
6 · The paper itself

Abstract

Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allow an earlier administration of a more appropriate antibiotic and could improve the outcome of these patients. The aim of this study was to develop a rapid protocol to identify the main microorganisms involved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples. First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL), endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP for

Indexed as

critical carediagnostic techniqueshospital-acquired pneumonia (HAP)loop-mediated isothermal amplification (LAMP)respiratory system

Identifiers

PMID31940771
PMCPMC7022425
OpenAlexW2998758160

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.