ArticlePloS one2020
Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 213 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
213 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Anorexia nervosa and microbiota: systematic review and critical appraisal.Eating and weight disorders : EWD · 2023Pooled it
- A systematic review of outdoor airborne fungal spore seasonality across Europe and the implications for health.The Science of the total environment · 2022Pooled it
- Chenopodium Quinoa's Ingredients Contribute to the Gut Microbiota's Metabolic Adaptations on Carbohydrate Metabolism.Plant foods for human nutrition (Dordrecht, Netherlands) · 2024Trial
- Quantitative analysis of the effects of essential oil mouthrinses on clinical plaque microbiome: a parallel-group, randomized trial.BMC oral health · 2024Trial
- Phage-microbe dynamics after sterile faecal filtrate transplantation in individuals with metabolic syndrome: a double-blind, randomised, placebo-controlled clinical trial assessing efficacy and safety.Nature communications · 2023Trial
- Gut microbiota predicts body fat change following a low-energy diet: a PREVIEW intervention study.Genome medicine · 2022Trial
- Examining the relationship between maternal body size, gestational glucose tolerance status, mode of delivery and ethnicity on human milk microbiota at three months post-partum.BMC microbiology · 2020Trial
- Article
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- Use of Fecal Sampling for Assessing Probiotic Effects on the Gut Microbiome: A Focused Narrative Review.Cureus · 2026Review
- The calf holobiont under challenge: longitudinal microbiota-pathogen dynamics and respiratory health.Veterinary research · 2026Article
- Single-cell detection and quantification of the microbiota by MicFLY.Cell host & microbe · 2026Article
- Intermittent Fasting Amplifies Gut-Endocrine Axis Responses to Senna alexandrina Supplementation in Obese Rats.Molecular nutrition & food research · 2026Article
- The Effect of Zinc Gluconate Supplementation on the Intestinal Microbiota and Growth Performance of Yorkshire Pigs.Animals : an open access journal from MDPI · 2026Article
- Neonatal intensive care unit exposures reprogram microbiome-metabolome trajectories and modulate host calprotectin in preterm infants: a longitudinal multi-omics study.NPJ biofilms and microbiomes · 2026Article
- Interactions of PGPR from the phylum bacillota with native rhizosphere microbiota: current insights and future perspectives.World journal of microbiology & biotechnology · 2026Review
- Mucin Type Drives Composition and Mucin Glycan Degradation of an In Vitro Synthetic Microbial Community.Environmental microbiology · 2026Article
- Navigating the translational labyrinth of the gut microbiota.Scientific reports · 2026Article
- Amplification Efficiency of Quantitative PCR Reactions is Improved by Addition of Non-Target DNA.Microbial ecology · 2026Article
- Standardizing oral microbiome sampling for qPCR: methodological and exploratory insights into nutritional status.Scientific reports · 2026Article
153 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of relative abundance data from next generation sequencing (NGS) can lead to misinterpretations of microbial community structures, as the increase of one taxon leads to the concurrent decrease of the other(s) in compositional data. Although different DNA- and cell-based methods as well as statistical approaches have been developed to overcome the compositionality problem, and the biological relevance of absolute bacterial abundances has been demonstrated, the human microbiome research has not yet adopted these methods, likely due to feasibility issues. Here, we describe how quantitative PCR (qPCR) done in parallel to NGS library preparation provides an accurate estimation of absolute taxon abundances from NGS data and hence provides an attainable solution to compositionality in high-throughput microbiome analyses. The advantages and potential challenges of the method are also discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.