ArticleNanoscale2020
In vivo deep-tissue microscopy with UCNP/Janus-dendrimers as imaging probes: resolution at depth and feasibility of ratiometric sensing.
Article in Nanoscale, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Imparting Biodegradability to Highly-Efficient Upconversion Nanoparticles via Facet-Selective Zirconium Doping.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Construction of a near-infrared excited RhB@UCNPs fluorescent probe for hypochlorite detection.Mikrochimica acta · 2025Article
- Unlocking Multimodal Nonlinear Microscopy for Deep-Tissue Imaging under Continuous-Wave Excitation with Tunable Upconverting Nanoparticles.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Recent advances of lanthanide nanomaterials in Tumor NIR fluorescence detection and treatment.Materials today. Bio · 2023Review
- Upconversion nanoparticle-based fluorescence resonance energy transfer sensing platform for the detection of cathepsin B activity in vitro and in vivo.Mikrochimica acta · 2023Article
- Properties and Bioapplications of Amphiphilic Janus Dendrimers: A Review.Pharmaceutics · 2023Review
- Bioimaging with Upconversion Nanoparticles.Advanced photonics research · 2022Article
- Biomedical Applications of Lanthanide Nanomaterials, for Imaging, Sensing and Therapy.Nanotheranostics · 2022Review
- Prospects for the Use of Upconverting Nanoparticles as a Contrast Agent for Enumeration of Circulating Cells in vivo.International journal of nanomedicine · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Lanthanide-based upconverting nanoparticles (UCNPs) are known for their remarkable ability to convert near-infrared energy into higher energy light, offering an attractive platform for construction of biological imaging probes. Here we focus on in vivo high-resolution microscopy - an application for which the opportunity to carry out excitation at low photon fluxes in non-linear regime makes UCNPs stand out among all multiphoton probes. To create biocompatible nanoparticles we employed Janus-type dendrimers as surface ligands, featuring multiple carboxylates on one 'face' of the molecule, polyethylene glycol (PEG) residues on another and Eriochrome Cyanine R dye as the core. The UCNP/Janus-dendrimers showed outstanding performance as vascular markers, allowing for depth-resolved mapping of individual capillaries in the mouse brain down to a remarkable depth of ∼1000 μm under continuous wave (CW) excitation with powers not exceeding 20 mW. Using a posteriori deconvolution, high-resolution images could be obtained even at high scanning speeds in spite of the blurring caused by the long luminescence lifetimes of the lanthanide ions. Secondly, the new UCNP/dendrimers allowed us to evaluate the feasibility of quantitative analyte imaging in vivo using a popular ratiometric UCNP-to-ligand excitation energy transfer (EET) scheme. Our results show that the ratio of UCNP emission bands, which for quantitative sensing should respond selectively to the analyte of interest, is also strongly affected by optical heterogeneities of the medium. On the other hand, the luminescence decay times of UCNPs, which are independent of the medium properties, are modulated via EET only insignificantly. As such, quantitative analyte sensing in biological tissues with UCNP-based probes still remains a challenge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.