ArticleAsian journal of andrology
Fluorescent probes for the detection of reactive oxygen species in human spermatozoa.
Article in Asian journal of andrology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Reactive Oxygen and Nitrogen Species in Male Reproductive Health: From Molecular Mechanisms to Clinical Consequences.Antioxidants (Basel, Switzerland) · 2026Review
- Spontaneous Non-Catalyzed Molecular Reactions and Interactions in the Human Body: Biomedical Implications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Oxidative Stress, Sperm DNA Fragmentation, or Both? Optimizing Test Selection in Male Infertility Evaluation.Antioxidants (Basel, Switzerland) · 2026Review
- Challenges in the standardization of innate immune status assessment.Frontiers in immunology · 2026Review
- Oxidative Damage Under Microgravity Conditions: Response Mechanisms, Monitoring Methods and Countermeasures on Somatic and Germ Cells.International journal of molecular sciences · 2025Review
- The Effects of Adding Hempseed Cake on Sperm Traits, Body Weight, Haematological and Biochemical Parameters in Rabbit Males.Veterinary sciences · 2024Article
- Quantitative Assessment of Intracellular Effectors and Cellular Response in RAGE Activation.Archives of internal medicine research · 2024Article
- The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment.Frontiers in veterinary science · 2024Article
- Effects of Benzo[a]pyrene on Human Sperm Functions: An In Vitro Study.International journal of molecular sciences · 2023Article
- Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models,Antioxidants (Basel, Switzerland) · 2023Article
- A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen.Heliyon · 2022Article
- Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.Nature communications · 2022Article
- Is oxidative stress evaluated in viable human spermatozoa a marker of good semen quality?Frontiers in endocrinology · 2022Article
- Impact of Lhcx2 on Acclimation to Low Iron Conditions in the DiatomFrontiers in plant science · 2022Article
- Article
- Mitochondrial metabolism determines the functional status of human sperm and correlates with semen parameters.Frontiers in cell and developmental biology · 2022Article
- Improved Anticancer Activities of a New Pentafluorothio-Substituted Vorinostat-Type Histone Deacetylase Inhibitor.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Male subfertility and oxidative stress.Redox biology · 2021Review
- Anticancer Activity and Mechanisms of Action of New Chimeric EGFR/HDAC-Inhibitors.International journal of molecular sciences · 2021Article
- Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males.Antioxidants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state, a requirement for functional spermatozoa. However, an increase in oxidative stress, due to an abnormal production of ROS, has been shown to be related to loss of sperm function, highlighting the importance of an accurate detection of sperm ROS, given the specific nature of this cell. In this work, we tested a variety of commercially available fluorescent probes to detect ROS and reactive nitrogen species (RNS) in human sperm, to define their specificity. Using both flow cytometry (FC) and fluorescence microscopy (FM), we confirmed that MitoSOX™ Red and dihydroethidium (DHE) detect superoxide anion (as determined using antimycin A as a positive control), while DAF-2A detects reactive nitrogen species (namely, nitric oxide). For the first time, we also report that RedoxSensor™ Red CC-1, CellROX
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.