Evidence map›Paper›PMID 31939177›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Methods for Studying TNFα-Induced Autophagy.

Sheyda Najafi, Ehab M Abo-Ali, Vikas V Dukhande

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sheyda NajafiDepartment of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA.
Ehab M Abo-AliDepartment of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA.
Vikas V DukhandeDepartment of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA. dukhandv@stjohns.edu.
St. John's University · US

Funding

Clues from a rare neurodegenerative disease towards target identification for diabetes mellitusSC2GM125550 · NIGMS · ST. JOHN'S UNIVERSITY · PI DUKHANDE, VIKAS VASUDEO · 2018 to 2020
$492k
NIGMS NIH HHS SC2 GM125550
6 · The paper itself

Abstract

Autophagy is an evolutionarily conserved cellular mechanism in eukaryotes that plays an important role in the maintenance of cellular homeostasis. The autophagy process maintains protein homeostasis by recycling damaged organelles and degrading many long-lived proteins in conjunction with the ubiquitin-proteasome system. Cytokines are low-molecular-weight secreted proteins that regulate a broad range of biological activities. For instance, pro-inflammatory cytokines such as tumor necrosis factor-α (TNFα) induce inflammation, autophagy, and apoptotic cell death. In this chapter, we discuss experimental techniques such as immunoblotting and fluorescence microscopy that can be utilized to measure autophagy in response to TNFα treatment.

Indexed as

AutophagyBiological AssayCell Line, TumorCytokinesHumansMicroscopy, FluorescenceProteasome Endopeptidase ComplexTumor Necrosis Factor-alphaUbiquitinCytokinesProteasome Endopeptidase ComplexTumor Necrosis Factor-alphaUbiquitinApoptosisAutophagyBafilomycin A1ChloroquineFluorescence microscopyImmunoblottingLC3p62TNFα

Identifiers

PMID31939177
PMCPMC9413520
OpenAlexW3000436908

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.