ArticleInternational journal of clinical and experimental pathology2018
The reactivation of P53 by saRNA affects the biological behavior in vitro in gastric cancer cells.
Article in International journal of clinical and experimental pathology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
- Small Activating RNAs: A Curated Database and an Overview of Rational Design Principles.Molecules (Basel, Switzerland) · 2026Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study sought to verify the reactivation effect of dsP53-285 that can up-regulate P53 expression in vitro. In addition, we explored the reactivation effect that dsP53-285 has on the biological behavior of gastric cancer cells. The specific small activating RNA (saRNA), dsP53-285, targeting the P53 gene promoter was synthesized. Also, a double strained control RNA (dsControl) was synthesized as a negative control, and then siP53 was synthesized to exclude the off-target effect. Both BGC-823 and MGC-803 cells were transfected with the corresponding microRNA, or just treated with lipofectamine2000. RT-qPCR and Western blot were adopted to detect P53 mRNA or the protein content of each group. CCK-8 was adopted to detect the proliferation of each group. The migration ability was assessed using the scratch-wound assay. The results of RT-qPCR and Western blot showed that dsP53-285 caused a significant up-regulation of the P53 gene (P<0.01), and the expression level of the P21 gene changed with the reactivation. The CCK-8 showed that, compared to the control group, the proliferation ability of the dsP53-285 group was inhibited significantly (P<0.01). The reactivation effect was in a time-course manner. The wound scratch assay proved that, compared to the control group, the migration ability of dsP53-285 group was inhibited significantly (P<0.01). This phenomenon provides a theoretical basis for the carcinostatic activity of small activating RNA (saRNA) and might indicate a new targeted treatment option for gastric cancer.
Indexed as
Identifiers
31938445PMC6958090W3015043087What OpenQuestion holds
Registered trials
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