Distribution changes of interstitial cells of Cajal during cholesterol gallstone formation in guinea pigs fed a high cholesterol diet.

backgroundCholesterol gallstone is commonly observed in patients with gallbladder disorders. Interstitial cells of Cajal (ICCs) in the gallbladder are important for regulating gallbladder motility and have a close relationship with cholelithiasis.

aimThe aim of this study was to explore changes in the distribution of gallbladder ICCs during cholesterol gallstone formation. MATERIALS AND

methodsThirty guinea pigs were randomly divided into three groups: the control group and study groups. Animals in study groups were fed on high cholesterol diet for 4 weeks or 8 weeks. Animals in the control groups were fed on a standard diet for 8 weeks. Immunohistochemistry was performed to observe the shape, size, morphology, and numbers of ICCs from the neck of the gallbladder to the fundus of the gallbladder, and terminal deoxynucleotidyl transferase dUTP nick-end labeling was performed to detect apoptosis in ICCs from the upper part of the gallbladder to the lower part of the gallbladder.

resultsThere were no differences in the shape, size, and morphology of the gallbladder ICCs in all groups. Cholesterol gallstones formed in guinea pigs fed on high cholesterol diet. The numbers of gallbladder ICCs were significantly decreased from the neck of the gallbladder to the fundus of the gallbladder, and gallbladder ICC apoptosis was significantly increased from the upper part of the gallbladder to the lower part of the gallbladder in both guinea pigs fed on high cholesterol diet (all

conclusionCholesterol gallstone formation reduced the density of gallbladder ICCs and increased the frequency of apoptotic gallbladder ICCs from the neck of the gallbladder to the fundus of the gallbladder, and these alterations may affect gallbladder ICC function.