ArticleNature communications2020
Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Molecular Mechanisms and Therapeutic Potential of Degron-Mediated Proteostasis Regulation in Neurodegenerative Diseases.Cellular and molecular neurobiology · 2026Review
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Article
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes.Biophysical journal · 2024Article
- Photoswitching the fluorescence of nanoparticles for advanced optical applications.Chemical science · 2024Review
- Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag.Communications biology · 2024Article
- Article
- Engineering Oncolytic Coxsackievirus A21 with Small Transgenes and Enabling Cell-Mediated Virus Delivery by Integrating Viral cDNA into the Genome.Journal of virology · 2023Article
- Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation.Nature chemistry · 2023Article
- Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.Journal of the American Chemical Society · 2023Article
- Outer Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in LiveBiosensors · 2023Article
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Article
- Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.Nature communications · 2021Article
- New Orange Ligand-Dependent Fluorescent Reporter for Anaerobic Imaging.ACS chemical biology · 2021Article
- Transient Fluorescence Labeling: Low Affinity-High Benefits.International journal of molecular sciences · 2021Review
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable kinetics and low background under Epi illumination. The on- and off-switching rates are controlled by the concentration of the ligand and the excitation light intensity, respectively, where the dissolved oxygen also promotes the off-switching. The photo-oxidation reaction mechanism of bilirubin in UnaG suggests that the lack of ligand-protein covalent bond allows the oxidized ligand to detach from the protein, emptying the binding cavity for rebinding to a fresh ligand molecule. We demonstrate super-resolution single-molecule localization imaging of various subcellular structures genetically encoded with UnaG, which enables facile labeling and simultaneous multicolor imaging of live cells. UnaG has the promise of becoming a default protein for high-performance super-resolution imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.