Evidence map›Paper›PMID 31937754›Full record

ArticleNature communications2020

Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes.

Xing Duan, Yizeng Li, Kexi Yi, Fengli Guo, HaiYang Wang, Pei-Hsun Wu, Jing Yang, Devin B Mair, Edwin Angelo Morales, Petr Kalab and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed, 1 pooled it
5.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.

  1. Endoplasmic reticulum in oocytes: spatiotemporal distribution and function.Journal of assisted reproduction and genetics · 2023
    Pooled it
  2. From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
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  18. Spatiotemporal Distribution and Function of Mitochondria in Oocytes.Reproductive sciences (Thousand Oaks, Calif.) · 2024
    Review
  19. The multiple links between actin and mitochondria.Nature reviews. Molecular cell biology · 2023
    Review
  20. Tubulin post-translational modifications in meiosis.Seminars in cell & developmental biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Xing DuanCenter for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD, 21205, USA.
Yizeng LiDepartment of Mechanical Engineering, Kennesaw State University, Marietta, GA, 30060, USA.ORCID http://orcid.org/0000-0002-3120-727X
Kexi YiStowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO, 64110, USA.
Fengli GuoStowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO, 64110, USA.
HaiYang WangCenter for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD, 21205, USA.
Pei-Hsun WuDepartment of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.ORCID http://orcid.org/0000-0002-7371-2960
Jing YangDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Devin B MairCenter for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD, 21205, USA.
Edwin Angelo MoralesCenter for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD, 21205, USA.
Petr KalabDepartment of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.ORCID http://orcid.org/0000-0002-8145-7728
Denis WirtzDepartment of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.ORCID http://orcid.org/0000-0001-6147-3045
Sean X SunDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.ORCID http://orcid.org/0000-0002-9077-7088
Rong LiCenter for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD, 21205, USA. rong@jhu.edu.ORCID http://orcid.org/0000-0002-2428-6061
Johns Hopkins University · USStowers Institute for Medical Research · US

Funding

The Role of Physical Cues in Collective Cell InvasionU54CA210173 · NCI · JOHNS HOPKINS UNIVERSITY · PI SUN, SEAN X · 2016 to 2020
$9.9M
Asymmetric Meiotic Cell Division of Mammalian OocytesR01HD086577 · NICHD · JOHNS HOPKINS UNIVERSITY · PI LI, RONG · 2016 to 2019
$1.4M
Acquisition of a PicoQuant FLIM SystemS10OD020007 · OD · JOHNS HOPKINS UNIVERSITY · PI MCCAFFERY, J. MICHAEL · 2016 to 2016
$231k
NCI NIH HHS U54 CA210173NICHD NIH HHS R01 HD086577NIH HHS S10 OD020007
6 · The paper itself

Abstract

Migration of meiosis-I (MI) spindle from the cell center to a sub-cortical location is a critical step for mouse oocytes to undergo asymmetric meiotic cell division. In this study, we investigate the mechanism by which formin-2 (FMN2) orchestrates the initial movement of MI spindle. By defining protein domains responsible for targeting FMN2, we show that spindle-periphery localized FMN2 is required for spindle migration. The spindle-peripheral FMN2 nucleates short actin bundles from vesicles derived likely from the endoplasmic reticulum (ER) and concentrated in a layer outside the spindle. This layer is in turn surrounded by mitochondria. A model based on polymerizing actin filaments pushing against mitochondria, thus generating a counter force on the spindle, demonstrated an inherent ability of this system to break symmetry and evolve directional spindle motion. The model is further supported through experiments involving spatially biasing actin nucleation via optogenetics and disruption of mitochondrial distribution and dynamics.

Indexed as

MeiosisActinsAnimalsAsymmetric Cell DivisionCytoplasmic VesiclesEndoplasmic ReticulumFemaleForminsMiceMitochondriaModels, BiologicalNerve Tissue ProteinsOocytesOrganellesProtein DomainsSequence DeletionActinsformin 2 protein, mouseForminsNerve Tissue Proteins

Identifiers

PMID31937754
PMCPMC6959240
OpenAlexW3000526160

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.