ArticleGenes2020
Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing.
Article in Genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- Development of a high-resolution multiplex qPCR method to profile microbial consortia in spaceflight water recovery systems.Biofilm · 2026Article
- Reactivation and Management of Endogenous Latent Herpesviruses in the Spaceflight Environment.Current microbiology · 2026Review
- Real-time analysis and visualization of nanopore metagenomic samples with MARTi.Genome research · 2025Article
- Pharmaceutical and biomedical challenges for crew autonomy in health preservation during future exploration missions.Communications medicine · 2025Review
- Computational Tools and Resources for Long-read Metagenomic Sequencing Using Nanopore and PacBio.Genomics, proteomics & bioinformatics · 2025Review
- Article
- Leveraging long-read sequencing technologies for pharmacogenomic testing: applications, analytical strategies, challenges, and future perspectives.Frontiers in genetics · 2025Review
- Microbiology of human spaceflight: microbial responses to mechanical forces that impact health and habitat sustainability.Microbiology and molecular biology reviews : MMBR · 2024Review
- Release of CD36-associated cell-free mitochondrial DNA and RNA as a hallmark of space environment response.Nature communications · 2024Article
- Space aquatic chemistry: A roadmap for drinking water treatment in microgravity.Environmental science and ecotechnology · 2024Article
- Validation of Cell-Free Protein Synthesis Aboard the International Space Station.ACS synthetic biology · 2024Article
- Article
- The newest Oxford Nanopore R10.4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling.Applied and environmental microbiology · 2023Article
- Nanopore sequencing technology and its applications.MedComm · 2023Review
- Genome Sequences of Bacteria Isolated from the International Space Station Water Systems.Microbiology resource announcements · 2023Article
- The Development of a 3D Printer-Inspired, Microgravity-Compatible Sample Preparation Device for Future Use Inside the International Space Station.Micromachines · 2023Article
- SituSeq: an offline protocol for rapid and remote Nanopore 16S rRNA amplicon sequence analysis.ISME communications · 2023Article
- Portable nanopore-sequencing technology: Trends in development and applications.Frontiers in microbiology · 2023Review
- Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.Cell reports methods · 2022Review
- Routine omics collection is a golden opportunity for European human research in space and analog environments.Patterns (New York, N.Y.) · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that could be conducted entirely aboard the International Space Station (ISS). Initial experiments demonstrated the process with a microbial mock community standard. The DNA was successfully amplified, primers were degraded, and libraries prepared and sequenced. The median percent identities for both datasets were 84%, as assessed from alignment of the mock community. The ability to correctly identify the organisms in the mock community standard was comparable for the sequencing data obtained in flight and on the ground. To validate the process on microbes collected from and cultured aboard the ISS, bacterial cells were selected from a NASA Environmental Health Systems Surface Sample Kit contact slide. The locations of bacterial colonies chosen for identification were labeled, and a small number of cells were directly added as input into the sequencing workflow. Prepared DNA was sequenced, and the data were downlinked to Earth. Return of the contact slide to the ground allowed for standard laboratory processing for bacterial identification. The identifications obtained aboard the ISS,
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.