ArticleInternational journal of molecular sciences2020
Effect of F-actin and Microtubules on Cellular Mechanical Behavior Studied Using Atomic Force Microscope and an Image Recognition-Based Cytoskeleton Quantification Approach.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Acute Thermal Elevation Alters Cellular Metabolic Activity, Redox Balance, and Iron Homeostasis in Butterfly Lizards (Animals : an open access journal from MDPI · 2026Article
- Quantifying intracellular mechanosensitive response upon spatially defined mechano-chemical triggering.eLife · 2026Article
- The Electromechanical Connectome: Integrating Voltage, Mechanical Nano-Forces, and Subcellular Fluid Phase Dynamics in Human Neural Computation.International journal of molecular sciences · 2026Review
- The Baraitser-Winter Cerebrofrontofacial Syndrome Recurrent R196H Variant in Cytoplasmic β-Actin Impairs Its Cellular Polymerization and Stability.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- The Protonic Brain: Nanoscale pH Dynamics, Proton Wires, and Acid-Base Information Coding in Neural Tissue.International journal of molecular sciences · 2026Review
- From Pixels to Patterns: A Multidimensional Framework to Decode Cytoskeletal Organization.Computational and structural biotechnology journal · 2026Review
- Vincristine Treatment Protects Against Podocyte Damage in Focal Segmental Glomerulosclerosis.Kidney international reports · 2025Article
- Fumonisin B1 Exerts Immunosuppressive Effects Through Cytoskeleton Remodeling and Function Attenuation of Mature Dendritic Cells.International journal of molecular sciences · 2025Article
- A novel computational approach to dissect the cytoskeletal architecture of cancer cells with invasive potential.Scientific reports · 2025Article
- Mechanical stress-induced autophagy is cytoskeleton dependent.Cell proliferation · 2024Article
- Article
- Applications of mass spectrometry imaging in botanical research.Advanced biotechnology · 2024Review
- Random Lasing for Bimodal Imaging and Detection of Tumor.Biosensors · 2023Article
- Long term culturing of CHO cells: phenotypic drift and quality attributes of the expressed monoclonal antibody.Biotechnology letters · 2023Article
- ILEE: Algorithms and toolbox for unguided and accurate quantitative analysis of cytoskeletal images.The Journal of cell biology · 2023Article
- Quantifying cytoskeletal organization from optical microscopy data.Frontiers in cell and developmental biology · 2023Review
- Modified protocol for culturingSTAR protocols · 2022Article
- Finite element analysis of the influence of cyclic strain on cells anchored to substrates with varying properties.Medical & biological engineering & computing · 2022Article
- Biomechanical Properties of Cancer Cells.Cells · 2021Review
- Nanomechanics in Monitoring the Effectiveness of Drugs Targeting the Cancer Cell Cytoskeleton.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoskeleton morphology plays a key role in regulating cell mechanics. Particularly, cellular mechanical properties are directly regulated by the highly cross-linked and dynamic cytoskeletal structure of F-actin and microtubules presented in the cytoplasm. Although great efforts have been devoted to investigating the qualitative relation between the cellular cytoskeleton state and cell mechanical properties, comprehensive quantification results of how the states of F-actin and microtubules affect mechanical behavior are still lacking. In this study, the effect of both F-actin and microtubules morphology on cellular mechanical properties was quantified using atomic force microscope indentation experiments together with the proposed image recognition-based cytoskeleton quantification approach. Young's modulus and diffusion coefficient of NIH/3T3 cells with different cytoskeleton states were quantified at different length scales. It was found that the living NIH/3T3 cells sense and adapt to the F-actin and microtubules states: both the cellular elasticity and poroelasticity are closely correlated to the depolymerization degree of F-actin and microtubules at all measured indentation depths. Moreover, the significance of the quantitative effects of F-actin and microtubules in affecting cellular mechanical behavior is depth-dependent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.