ArticleSensors (Basel, Switzerland)2020
Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux.
Article in Sensors (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Review
- Determination of pepsin in human saliva using pepsin-susceptible peptide reporter and colorimetric dipstick assay: a prospective, cross-sectional study.Mikrochimica acta · 2024Article
- Salivary diagnostics: opportunities and challenges.Theranostics · 2024Review
- Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation.International journal of oral science · 2023Review
- Pretreatment Methods for Human Nasopharyngeal Swabs to Increase the Signal to Noise Ratio of High Sensitivity Immunoassays.ACS measurement science au · 2022Article
- Electrochemical detection of uric acid in undiluted human saliva using uricase paper integrated electrodes.Scientific reports · 2022Article
- Investigation of Whole and Glandular Saliva as a Biomarker for Alzheimer's Disease Diagnosis.Brain sciences · 2022Article
- [The effect of expression of pepsin in vocal polyps on postoperative curative effect].Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2022Article
- Multitime point pepsin testing can double the rate of the diagnosis of laryngopharyngeal reflux.Laryngoscope investigative otolaryngology · 2021Article
- How I Approach Laryngopharyngoesophageal Reflux (LPR).Current gastroenterology reports · 2021Review
- Sorafenib Repurposing for Ophthalmic Delivery by Lipid Nanoparticles: A Preliminary Study.Pharmaceutics · 2021Article
- Pepsin and Laryngeal and Hypopharyngeal Carcinomas.Clinical and experimental otorhinolaryngology · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Salivary pepsin is a promising marker for the non-invasive diagnosis of laryngopharyngeal reflux (LPR). For reliable results regarding pepsin in saliva, it is critical to standardize the collection, storage, and pre-processing methods. In this study, we optimized the saliva collection protocols, including storage conditions, i.e., solution, temperature, and time, and the pre-processing filter for pepsin. Moreover, we prepared a simple immunochromatographic strip for the rapid detection of pepsin and evaluated its sensing performance. As a result, we selected a polypropylene (PP) filter as the pre-processing filter for salivary pepsin in low resource settings, such as those where point of care testing (POCT) is conducted. This filter showed a similar efficiency to the centrifuge (standard method). Finally, we detected the pepsin using gold nanoparticles conjugated with monoclonal pepsin antibody. Under optimized conditions, the lower limit of detection for pepsin test strips was determined as 0.01 μg/mL. Furthermore, we successfully detected the salivary pepsin in real saliva samples of LPR patients, which were pre-processed by the PP filter. Therefore, we expect that our saliva collection protocol and pepsin immunochromatographic strip can be utilized as useful tools for a non-invasive diagnosis/screening of LPR in POCT.
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