Evidence map›Paper›PMID 31934601›Full record

ArticleStructural dynamics (Melville, N.Y.)2020

Best practices for high data-rate macromolecular crystallography (HDRMX).

Herbert J Bernstein, Lawrence C Andrews, Jorge A Diaz, Jean Jakoncic, Thu Nguyen, Nicholas K Sauter, Alexei S Soares, Justin Y Wei, Maciej R Wlodek, Mario A Xerri

Abstract read
In one paragraph

Article in Structural dynamics (Melville, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Scotty: lattice coincidences for macromolecular crystallographic phasing.Acta crystallographica. Section D, Structural biology · 2026
    Article
  2. Macromolecular crystallography at SPring-8 and SACLA.Journal of synchrotron radiation · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Approximating lattice similarity.Acta crystallographica. Section A, Foundations and advances · 2023
    Article
  7. Article
  8. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021
    Review
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Herbert J BernsteinRonin Institute for Independent Scholarship, c/o NSLS-II Bldg 745, Brookhaven National Laboratory, Upton, New York 11973, USA.ORCID https://orcid.org/0000-0002-0517-8532
Lawrence C AndrewsRonin Institute for Independent Scholarship, 9515 NE 137th St., Kirkland, Washington 98034, USA.ORCID https://orcid.org/0000-0002-4451-1641
Jorge A DiazRonin Institute for Independent Scholarship, c/o NSLS-II Bldg 745, Brookhaven National Laboratory, Upton, New York 11973, USA.
Jean JakoncicBrookhaven National Laboratory, NSLS-II Bldg 745, Upton, New York 11973, USA.
Thu NguyenStony Brook University, Stony Brook, New York 11794, USA.
Nicholas K SauterLawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, California 94720, USA.
Alexei S SoaresBrookhaven National Laboratory, NSLS-II Bldg 745, Upton, New York 11973, USA.ORCID https://orcid.org/0000-0002-6565-8503
Justin Y WeiMount Sinai High School, 110 N Country Rd., Mt Sinai, New York 11766, USA.ORCID https://orcid.org/0000-0002-2480-5183
Maciej R WlodekStony Brook University, Stony Brook, New York 11794, USA.
Mario A XerriMount Sinai High School, 110 N Country Rd., Mt Sinai, New York 11766, USA.

Funding

X-ray Scattering Technology CoreP30GM133893 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI Sean McSweeney · 2019 to 2026
$38.6M
X-RAY STRUCTURE OF RIFM PROTEIN FROM THE BIOSYNTHESIS PATHWAY OF THE ANSAMYCIN AP41RR012408 · NCRR · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI ORVILLE, ALLEN M · 1998 to 2011
$32.2M
Life Science and Biomedical technology Research center at NSLS-II LSBRP41GM111244 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MCSWEENEY, SEAN · 2014 to 2018
$16.4M
DIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1M
Macromolecular Crystallography at the National Synchrotron Light SourceP41GM103473 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI SWEET, ROBERT M · 2012 to 2013
$4.9M
Detecting elusive biologically significant structural differences with serial crystallographyR21GM129570 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI SOARES, ALEXEI SUAREZ · 2018 to 2019
$410k
NCRR NIH HHS P41 RR012408NIGMS NIH HHS P30 GM133893NIGMS NIH HHS P41 GM103473NIGMS NIH HHS P41 GM111244NIGMS NIH HHS R01 GM117126NIGMS NIH HHS R21 GM129570
6 · The paper itself

Abstract

In macromolecular crystallography, higher flux, smaller beams, and faster detectors open the door to experiments with very large numbers of very small samples that can reveal polymorphs and dynamics but require re-engineering of approaches to the clustering of images both at synchrotrons and XFELs (X-ray free electron lasers). The need for the management of orders of magnitude more images and limitations of file systems favor a transition from simple one-file-per-image systems such as CBF to image container systems such as HDF5. This further increases the load on computers and networks and requires a re-examination of the presentation of metadata. In this paper, we discuss three important components of this problem-improved approaches to the clustering of images to better support experiments on polymorphs and dynamics, recent and upcoming changes in metadata for Eiger images, and software to rapidly validate images in the revised Eiger format.

Identifiers

PMID31934601
PMCPMC6952294

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.