ArticleNPJ Regenerative medicine2020
Differential functional roles of fibroblasts and pericytes in the formation of tissue-engineered microvascular networks in vitro.
Article in NPJ Regenerative medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Self-organization of vascularized muscle from bovine embryonic stem cells.Nature communications · 2026Article
- Strategies to Microengineer Vascularized, Mineralized, and Immunomodulatory Niches for Dental Regeneration.Journal of endodontics · 2026Article
- Protocol for fibrin-based endothelial-cardiomyocyte co-culture in a microfluidic device.STAR protocols · 2026Article
- Automating Vascular Biology: An End-to-End Automated Workflow for High-Throughput Blood Vessel-on-a-Chip Production and Multi-Site Validation.Advanced healthcare materials · 2026Article
- Advances in Modeling the Inner Blood-Retinal Barrier: From Static Tissue Cell Cultures to Microphysiological Systems.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Clickable PEG-norbornene microgels support suspension bioprinting and microvascular assembly.Acta biomaterialia · 2025Article
- Article
- Pericytes Promote More Vascularization than Stromal Cells via an Interleukin-6-Dependent Mechanism in Microfluidic Chips.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Biofabrication and Characterization of Vascularizing PEG-Norbornene Microgels.Journal of biomedical materials research. Part A · 2025Article
- Immune-Infiltrated Cancer Spheroid Model with Vascular Recirculation Reveals Temporally Dependent and Tissue-Specific Macrophage Recruitment.Advanced healthcare materials · 2025Article
- Stochastic to Deterministic: A Straightforward Approach to Create Serially Perfusable Multiscale Capillary Beds.ACS biomaterials science & engineering · 2025Article
- Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2024Article
- Adaptable Manufacturing and Biofabrication of Milliscale Organ Chips With Perfusable Vascular Beds.Biotechnology journal · 2024Article
- High-Scale 3D-Bioprinting Platform for the Automated Production of Vascularized Organs-on-a-Chip.Advanced healthcare materials · 2024Article
- Human Brain In Vitro Model for Pathogen Infection-Related Neurodegeneration Study.International journal of molecular sciences · 2024Review
- Cadherin Expression Is Regulated by Mechanical Phenotypes of Fibroblasts in the Perivascular Matrix.Cells, tissues, organs · 2024Article
- Lung epithelial-endothelial-mesenchymal signaling network with hepatocyte growth factor as a hub is involved in bronchopulmonary dysplasia.Frontiers in cell and developmental biology · 2024Review
- Flow in fetoplacental-like microvessels in vitro enhances perfusion, barrier function, and matrix stability.Science advances · 2023Article
- 3D printed elastomeric biomaterial mitigates compaction during in vitro vasculogenesis.Acta biomaterialia · 2023Article
- Emergent mechanical control of vascular morphogenesis.Science advances · 2023Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 2 countries.
Funding
Abstract
Formation of a perfusable microvascular network (μVN) is critical for tissue engineering of solid organs. Stromal cells can support endothelial cell (EC) self-assembly into a μVN, but distinct stromal cell populations may play different roles in this process. Here we describe the differential effects that two widely used stromal cell populations, fibroblasts (FBs) and pericytes (PCs), have on μVN formation. We examined the effects of adding defined stromal cell populations on the self-assembly of ECs derived from human endothelial colony forming cells (ECFCs) into perfusable μVNs in fibrin gels cast within a microfluidic chamber. ECs alone failed to fully assemble a perfusable μVN. Human lung FBs stimulated the formation of EC-lined μVNs within microfluidic devices. RNA-seq analysis suggested that FBs produce high levels of hepatocyte growth factor (HGF). Addition of recombinant HGF improved while the c-MET inhibitor, Capmatinib (INCB28060), reduced μVN formation within devices. Human placental PCs could not substitute for FBs, but in the presence of FBs, PCs closely associated with ECs, formed a common basement membrane, extended microfilaments intercellularly, and reduced microvessel diameters. Different stromal cell types provide different functions in microvessel assembly by ECs. FBs support μVN formation by providing paracrine growth factors whereas PCs directly interact with ECs to modify microvascular morphology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.