ArticleInternational journal of clinical and experimental pathology2019
MicroRNA-497-5p attenuates IL-1β-induced cartilage matrix degradation in chondrocytes via Wnt/β-catenin signal pathway.
Article in International journal of clinical and experimental pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Oxidative Stress, MicroRNAs, and Long Non-Coding RNAs in Osteoarthritis Pathogenesis: Cross-Talk and Molecular Mechanisms Involved.International journal of molecular sciences · 2025Review
- ITGB5 as a Potential Diagnostic Biomarker for Osteoarthritis.Journal of musculoskeletal & neuronal interactions · 2025Article
- Identification of Key Glycolysis-Related Genes in Osteoarthritis and Their Correlation with Immune Infiltration Using Bioinformatics Analysis and Machine Learning.Open access rheumatology : research and reviews · 2025Article
- Hypermethylation of microRNA-497-3p contributes to progression of thyroid cancer through activation of PAK1/β-catenin.Cell biology and toxicology · 2023Article
- Wnt3a knockdown promotes collagen type II expression in rat chondrocytes.Experimental and therapeutic medicine · 2022Article
- PRDX2 Knockdown Inhibits Extracellular Matrix Synthesis of Chondrocytes by Inhibiting Wnt5a/YAP1/CTGF and Activating IL-6/JAK2/STAT3 Pathways in Deer Antler.International journal of molecular sciences · 2022Article
- A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells.Applied biochemistry and biotechnology · 2022Article
- miRNA Let-7a-5p targets RNA KCNQ1OT1 and Participates in Osteoblast Differentiation to Improve the Development of Osteoporosis.Biochemical genetics · 2022Article
- Long Non-Coding RNA Cancer Susceptibility Candidate 9 Regulates the Malignant Biological Behavior of Nasopharyngeal Carcinoma Cells by Targeting miR-497-5p/Wnt3a/β-catenin Signaling Pathway.Frontiers in oncology · 2022Article
- Circular RNA MELK Promotes Chondrocyte Apoptosis and Inhibits Autophagy in Osteoarthritis by Regulating MYD88/NF-Contrast media & molecular imaging · 2022Article
- The Wnt signaling cascade in the pathogenesis of osteoarthritis and related promising treatment strategies.Frontiers in physiology · 2022Review
- Non-coding RNAs in ossification of the posterior longitudinal ligament.Frontiers in genetics · 2022Review
- Serum microRNAs in osteoporotic fracture and osteoarthritis: a genetic and functional study.Scientific reports · 2021Article
- The Interaction between microRNAs and the Wnt/β-Catenin Signaling Pathway in Osteoarthritis.International journal of molecular sciences · 2021Review
- MicroRNA-497-5p stimulates osteoblast differentiation through HMGA2-mediated JNK signaling pathway.Journal of orthopaedic surgery and research · 2020Article
- [The role of Wnt signaling pathway in osteoarthritis via the dual-targeted regulation of cartilage and subchondral bone].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Article
- Hydrostatic Pressure Regulates Oxidative Stress through microRNA in Human Osteoarthritic Chondrocytes.International journal of molecular sciences · 2020Article
- Construction of Potential miRNA-mRNA Regulatory Network in COPD Plasma by Bioinformatics Analysis.International journal of chronic obstructive pulmonary disease · 2020Article
- SPOCK1: a multi-domain proteoglycan at the crossroads of extracellular matrix remodeling and cancer development.American journal of cancer research · 2020Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteoarthritis (OA) is a degenerative joint disease. Degradation of extracellular matrix (ECM) in chondrocytes is closely related to joint destruction in OA progression. MicroRNAs (miRNAs) have been reported to play important roles in progression of OA. However, the roles of miR-497-5p in OA process and its underlying mechanism remain not been well established. Chondrocytes were obtained from articular cartilage and stimulated with IL-1β. The expression of miR-497-5p and Wnt3a was detected by qRT-PCR. Western blot analysis was performed to measure the proteins of Wnt3a, collagen II, aggrecan matrix metalloproteinase (MMP) 13 and ADAMTS4. Cell apoptosis was detected by flow cytometry. The putative binding sites of miR-497-5p and Wnt3a were predicted by Targetscan and verified through luciferase report assay. We found that miR-497-5p expression was reduced and Wnt3a expression was enhanced in OA cartilage and IL-1β-stimulated chondrocytes. Moreover, Wnt3a was a direct target of miR-497-5p, and expression of miR-497-5p was negatively correlated with Wnt3a level in OA cartilage. Furthermore, overexpression of miR-497-5p prominently increased the expression of cartilage matrix molecules collagen II and aggrecan, and reduced the expression of matrix-degrading enzymes MMP13 and ADAMTS4 while overexpression of Wnt3a reversed these effects, whereas addition of DKK-1attenuated the Wnt3a-mediated functions in IL-1β-stimulated chondrocytes. In conclusion, miR-497-5p attenuated IL-1β-induced cartilage matrix degradation in chondrocytes via Wnt/β-catenin signal pathway, providing a potential therapeutic target for treatment of OA.
Indexed as
Identifiers
31934153PMC6949713W3006546510What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.