ArticleInternational journal of clinical and experimental pathology2019
Effects of autophage on the proliferation and apoptosis of clear cell renal carcinoma 786-O cells.
Article in International journal of clinical and experimental pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 4 citations in OpenAlex.
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
objectiveAutophagy plays important roles in tumor occurrence and development. The present study aimed to investigate the association between autophagy and apoptosis in clear cell renal carcinoma cells (ccRCCs).
methodsAtg7-overexpressing and -knockdown RCC 786-O cells (pLenti6.3-ATG7 and sh-ATG7-2 lv) were established using lentiviral transfection and interference shRNA. pLenti6.3-GFP and sh-scramb-con lv were used as controls. Cells were cultured in medium with or without the apoptosis inhibitor Z-VAD-FMK. Cell apoptosis were detected by flow cytometry. Cell proliferation was determined by MTT assay. Expression of apoptotic pathway proteins was measured by Western blot.
resultsThe apoptosis rate of Z-VAD-FMK-treated cells was significantly decreased compared with untreated cells (P < 0.05). However, no significant difference in the apoptosis rate was detected among cell groups with different autophage level. The Z-VAD-FMK treatment induced significant changes in apoptosis rate in all cell groups, but only slightly changed the cell proliferation. When cell apoptosis were inhibited by Z-VAD-FMK, the cell viability in pLenti6.3-ATG7 group was significantly reduced compared with 786-O control (P < 0.05), whereas the cell viability in sh-ATG7-2 lv was significantly enhanced (P < 0.05) indicating that cell proliferation was closely associated with the level of autophage. The expression of caspase proteins in pLenti6.3-ATG7 was significantly higher compared with sh-ATG7-2 lv group (P < 0.05).
conclusionautophagy and apoptosis are independent processes of PCD in human ccRCC 786-O cells. Autophagy is the main type of PCD and may be closely associated with apoptosis through the classical death receptor, mitochondria and endoplasmic reticulum apoptosis pathway.
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Identifiers
31933948PMC6947043W3009237529What OpenQuestion holds
Registered trials
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