ArticleInternational journal of clinical and experimental pathology2019
Effect of miR-135b inhibitor on biological characteristics of osteosarcoma cells through up-regulating PPM1A.
Article in International journal of clinical and experimental pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- miR-135b: A key role in cancer biology and therapeutic targets.Non-coding RNA research · 2025Review
- MicroRNA-487a-3p inhibits the growth and invasiveness of oral squamous cell carcinoma by targeting PPM1A.Bioengineered · 2021Article
- [MiR-300 inhibits invasion and metastasis of osteosarcoma cell MG63 by negatively regulating PTTG1].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Leveraging Methylation Alterations to Discover Potential Causal Genes Associated With the Survival Risk of Cervical Cancer in TCGA Through a Two-Stage Inference Approach.Frontiers in genetics · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
miR-135b is expressed abnormally in various tumors and plays an important role in the occurrence and development of tumors bydifferent pathways. The role of miR-135b in osteosarcoma (OS) and its mechanisms were uncertain. The study aimed to clarify the role of miR-135b in osteosarcoma (OS) cells and explore the effect of miR-135b inhibitor on the biological characteristics of OS cells. Firstly, Compared with adjacent bone tissues, the expression of miR-135b was increased and inversely correlated with potential target-PPM1A mRNA, while the expression of PPM1A was decreased in OS tissues. Dual luciferase reporter assay was used to verify that PPM1A was a target gene of miR-135b and RT-PCR and western blot were used to detect that miR-135b negatively regulated expression of PPM1A in MG63 and U2OS cell lines. After transfection, miR-135b inhibitor significantly inhibited cell proliferation and invasion both in MG63 and U2OS cells and blocked cell cycle in the G2/M phase and induced cells apoptosis, while PPM1A knockdown abolished the inhibition of miR-135b inhibitor on the proliferation and invasion of OS cells. In conclusion, miR-135b is up-regulated in OS cells and down regulating miR-135b expression could inhibit the proliferation and invasion of OS cells by up-regulating PPM1A. miR-135b might be a new therapeutic target of OS.
Indexed as
Identifiers
31933876PMC6945162W3010186667What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.