Evidence map›Paper›PMID 31933178›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2020

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14.

Yunqiang Wu, Yanan Liu, Ke Dong, Qingtian Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Infectious medicine · 2023
    Review
  2. Frontiers in microbiology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Yunqiang WuDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, B06, Building 1, 280 South Chongqing Road, Shanghai, 200025, China.
Yanan LiuDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, B06, Building 1, 280 South Chongqing Road, Shanghai, 200025, China.
Ke DongDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. kiwidong@126.com.
Qingtian LiDepartment of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, B06, Building 1, 280 South Chongqing Road, Shanghai, 200025, China. qingtianli@sjtu.edu.cn.ORCID http://orcid.org/0000-0003-1170-0806
Shanghai Jiao Tong University · CN

Funding

National Natural Science Foundation of China 81201334
6 · The paper itself

Abstract

Antimicrobial peptides are considered to be one of the candidate antimicrobial agents for antibiotic-resistant bacterial infection in the future. The effects of antimicrobial peptide hBD3-CBD on Pseudomonas aeruginosa PA14 and PA14 ΔexsA were analyzed by the bactericidal effects, hemolysis assays, pyocyanin pigment productions, and virulence factor expressions (exoU, exoS, hcnA, and lasB). Pyocyanin production and virulence factor expressions are important features of the type III secretion system in Pseudomonas aeruginosa. HBD3-CBD killed PA14 and PA14 ΔexsA with similar efficiency; it lowered the hemolysis levels of PA14 and PA14 ΔexsA and reduced the pyocyanin production, biofilm formation, and exoU, exoS, and lasB expressions in PA14. Compared with PA14, PA14 ΔexsA showed a lower hemolysis effect, pyocyanin production, exoU, and lasB expressions. The effects of hBD3-CBD on the PA14 toxin secretion were similar to the changes in the type III secretion system mutant isolate PA14 ΔexsA. Our results demonstrated that the type III secretion system was involved in the biological functions on PA 14 from hBD3-CBD.

Indexed as

AnimalsBacterial Proteinsbeta-DefensinsBiofilmsCarbohydrate MetabolismCarbohydratesErythrocytesHemolysisHumansProtein BindingPseudomonas aeruginosaPseudomonas InfectionsPyocyanineSheepTrans-ActivatorsType III Secretion SystemsBacterial Proteinsbeta-DefensinsCarbohydratesExsA protein, bacteriaPyocyanineTrans-ActivatorsType III Secretion SystemsAntimicrobial peptidehBD3Pseudomonas aeruginosaT3SS

Identifiers

PMID31933178
PMCPMC7058721
OpenAlexW3000103975

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.