ArticleNature biotechnology2020
An integrated perfusion machine preserves injured human livers for 1 week.
Article in Nature biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 171 papers, 5 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
171 citing papers in PubMed, 5 syntheses or guidelines pooled it, 372 citations in OpenAlex.
- Integrating Dialysis in Ex Situ Machine Perfusion: A Systematic Review and Meta-Analysis of Outcomes.Artificial organs · 2026Pooled it
- Donor-Related Risk Factors for Normothermic Machine Perfusion in Liver Transplantation: A Meta-Analysis.Liver international : official journal of the International Association for the Study of the Liver · 2025Pooled it
- A meta-analysis of perfusion parameters affecting weight gain in ex vivo perfusion.Artificial organs · 2025Pooled it
- Oncologic Outcomes of Interventions to Decrease Allograft Ischemia-Reperfusion Injury within Patients Undergoing Liver Transplantation for Hepatocellular Carcinoma: A Systematic Review.Current oncology (Toronto, Ont.) · 2024Pooled it
- Ex vivo machine perfusion: current applications and future directions in liver transplantation.Langenbeck's archives of surgery · 2021Pooled it
- A clinically deployed dual-compartment biochemical monitoring platform for human liver perfusion.Nature communications · 2026Article
- Review
- From Preservation to Repair: A Systematic Review of Therapeutic Organ Rehabilitation During Normothermic Ex Vivo Machine Perfusion.Transplantation direct · 2026Review
- Hemodiafiltration improves performance of 24 hour ex situ normothermic liver machine perfusion.JHEP reports : innovation in hepatology · 2026Article
- Focal Hepatic Hypoperfusion After Normothermic Machine Perfusion of Liver Grafts Is Associated with a Higher Comprehensive Complication Index.Bioengineering (Basel, Switzerland) · 2026Article
- Acellular Normothermic Machine Perfusion Does not Exacerbate Acute Rejection in Rodent Vascularized Composite Allografts.Transplantation direct · 2026Article
- Digital twins of ex vivo human lungs enable accurate and personalized evaluation of therapeutic efficacy.Nature biotechnology · 2026Article
- Graft-derived VWF drives platelet activation and thrombocytopenia during porcine liver xenotransplantation to brain-dead human recipients.The Journal of clinical investigation · 2026Article
- Performance of Predefined Viability Criteria During Prolonged End-Ischemic Normothermic Perfusion of Brain-Dead Donor Liver Grafts.Clinical transplantation · 2026Article
- Normothermic perfusion of human livers for profiling lentiviral vector pharmacokinetics and transduction.Molecular therapy. Advances · 2026Article
- Improving surgical treatments for hepatocellular carcinoma.Nature reviews. Gastroenterology & hepatology · 2026Review
- Integration of multi-modal monitoring for dynamic control of large-scale 3D tissue bioreactors.Scientific reports · 2026Article
- Machine perfusion for liver transplantation: opportunities and challenges for the next generation of transplant surgeons.Frontiers in transplantation · 2026Review
- Time as a Therapeutic Ally: The Promise of Long-Term Solid Organ and Tissue Perfusion.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
- Integrating senotherapeutics into transplantation: liver reconditioning in an aging donor pool.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
111 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Commented on by
- Commented on by
- Commented on by
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to preserve metabolically active livers ex vivo for 1 week or more could allow repair of poor-quality livers that would otherwise be declined for transplantation. Current approaches for normothermic perfusion can preserve human livers for only 24 h. Here we report a liver perfusion machine that integrates multiple core physiological functions, including automated management of glucose levels and oxygenation, waste-product removal and hematocrit control. We developed the machine in a stepwise fashion using pig livers. Study of multiple ex vivo parameters and early phase reperfusion in vivo demonstrated the viability of pig livers perfused for 1 week without the need for additional blood products or perfusate exchange. We tested the approach on ten injured human livers that had been declined for transplantation by all European centers. After a 7-d perfusion, six of the human livers showed preserved function as indicated by bile production, synthesis of coagulation factors, maintained cellular energy (ATP) and intact liver structure.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.