Evidence map›Paper›PMID 31932632›Full record

ArticleNature communications2020

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.

Mikhail E Matlashov, Daria M Shcherbakova, Jonatan Alvelid, Mikhail Baloban, Francesca Pennacchietti, Anton A Shemetov, Ilaria Testa, Vladislav V Verkhusha

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.

0numbers the graph read from it
0cells of the map it votes in
116citing papers in PubMed
26.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

116 citing papers in PubMed, 220 citations in OpenAlex.

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  3. Bright monomeric fluorescent protein elite-niRFP704 for two-channel near-infrared STED nanoscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026
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56 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 3 countries.

Mikhail E MatlashovDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.ORCID http://orcid.org/0000-0003-0769-4749
Daria M ShcherbakovaDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.
Jonatan AlvelidDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0002-3554-9322
Mikhail BalobanDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.
Francesca PennacchiettiDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
Anton A ShemetovDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.
Ilaria TestaDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0003-4005-4997
Vladislav V VerkhushaDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA. vladislav.verkhusha@einsteinmed.org.ORCID http://orcid.org/0000-0002-2083-8121
Albert Einstein College of Medicine · USScience for Life Laboratory · SE

Funding

Near-Infrared Fluorescent Proteins, Biosensors and Optogenetic ToolsR35GM122567 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI Vladislav Verkhusha · 2017 to 2026
$4.3M
Calcium biosensors for deep-tissue imaging and spectral multiplexingU01NS103573 · NINDS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI VERKHUSHA, VLADISLAV · 2017 to 2019
$1.8M
A system for whole-brain recording and control of activated neurons with near-infrared lightR21EY030705 · NEI · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI SHCHERBAKOVA, DARIA · 2019 to 2019
$503k
NEI NIH HHS R21 EY030705NIGMS NIH HHS R35 GM122567NINDS NIH HHS U01 NS103573
6 · The paper itself

Abstract

Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to engineer a complete set of monomeric NIR FPs, which are the brightest genetically encoded NIR probes. We demonstrate that the enhanced miRFP series of NIR FPs, which combine high effective brightness in mammalian cells and monomeric state, perform well in both nanometer-scale imaging with diffraction unlimited stimulated emission depletion (STED) microscopy and centimeter-scale imaging in mice. In STED we achieve ~40 nm resolution in live cells. In living mice we detect ~10

Indexed as

AnimalsCell LineFemaleFluorescenceHumansIntravital MicroscopyLuminescent ProteinsMiceMolecular ImagingProtein EngineeringProtein StabilitySpectroscopy, Near-InfraredLuminescent Proteins

Identifiers

PMID31932632
PMCPMC6957686
OpenAlexW3000358775

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.