ArticleBMC anesthesiology2020
Neutrophil extracellular trap formation and nuclease activity in septic patients.
Article in BMC anesthesiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
- Liquid Biopsy in Neuropsychiatric Disorders: A Step Closer to Precision Medicine.Molecular neurobiology · 2025Review
- Deficient neutrophil responses early in influenza infection promote viral replication and pulmonary inflammation.PLoS pathogens · 2025Article
- Septic Hyperinflammation-Is There a Role for Extracorporeal Blood Purification Techniques?International journal of molecular sciences · 2024Review
- Impaired balance between neutrophil extracellular trap formation and degradation by DNases in COVID-19 disease.Journal of translational medicine · 2024Article
- Visualizing neutrophil extracellular traps in septic equine synovial and peritoneal fluid samples using immunofluorescence microscopy.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Targeting the Granulocytic Defense againstInternational journal of molecular sciences · 2023Article
- Preterm ETs Are Significantly Reduced Compared with Adults and Partially Reduced Compared with Term Infants.Children (Basel, Switzerland) · 2022Article
- Cytokine Storm-Definition, Causes, and Implications.International journal of molecular sciences · 2022Review
- Review
- Dynamic Changes in the Ability to Release Neutrophil ExtraCellular Traps in the Course of Childhood Acute Leukemias.International journal of molecular sciences · 2021Article
- The Role of Neutrophil Extracellular Traps in Cancer.Frontiers in oncology · 2021Review
- Sepsis-Pathophysiology and Therapeutic Concepts.Frontiers in medicine · 2021Review
- Use of Intravenous Immunoglobulins in Sepsis Therapy-A Clinical View.International journal of molecular sciences · 2020Review
- Immunesenescence: A Predisposing Risk Factor for the Development of COVID-19?Frontiers in immunology · 2020Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is little knowledge, whether in patients with sepsis neutrophil extracellular trap (NET) formation and NET degrading nuclease activity are altered. Thus, we tested the hypotheses that 1) NET formation from neutrophils of septic patients is increased compared to healthy volunteers, both without stimulation and following incubation with mitochondrial DNA (mtDNA), a damage-associated molecular pattern, or phorbol 12-myristate 13-acetate (PMA; positive control) and 2) that serum nuclease activities are increased as well.
methodsFollowing ethic committee approval, we included 18 septic patients and 27 volunteers in this prospective observational trial. Blood was withdrawn and NET formation from neutrophils was analyzed in vitro without stimulation and following incubation with mtDNA (10 μg/well) or PMA (25 nmol). Furthermore, serum nuclease activity was assessed using gel electrophoresis.
resultsIn contrast to our hypothesis, in septic patients, unstimulated NET release from neutrophils was decreased by 46.3% (4.3% ± 1.8 SD vs. 8.2% ± 2.9, p ≤ 0.0001) and 48.1% (4.9% ± 2.5 vs. 9.4% ± 5.2, p = 0.002) after 2 and 4 h compared to volunteers. mtDNA further decreased NET formation in neutrophils from septic patients (4.7% ± 1.2 to 2.8% ± 0,8; p = 0.03), but did not alter NET formation in neutrophils from volunteers. Of note, using PMA, as positive control, we ensured that neutrophils were still able to form NETs, with NET formation increasing to 73.2% (±29.6) in septic patients and 91.7% (±7.1) in volunteers (p = 0.22). Additionally, we show that serum nuclease activity (range: 0-6) was decreased in septic patients by 39.6% (3 ± 2 vs 5 ± 0, median and ICR, p = 0.0001) compared to volunteers.
conclusionsUnstimulated NET formation and nuclease activity are decreased in septic patients. mtDNA can further reduce NET formation in sepsis. Thus, neutrophils from septic patients show decreased NET formation in vitro despite diminished nuclease activity in vivo.
trial registrationDRKS00007694, german clinical trials database (DRKS). Retrospectively registered 06.02.2015.
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