ArticleBMC plant biology2020
Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 66 citations in OpenAlex.
- Rational gene stacking for seed-specific carbon partitioning to simultaneously enhance lipid and protein contents in Arabidopsis.Journal of experimental botany · 2026Article
- Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026Review
- Identification, evolutionary diversification, and stress response roles of SmLACSs in Salix matsudana.BMC genomics · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Multi-omics analyses reveal regulatory networks underpinning metabolite biosynthesis in Nicotiana tabacum.Nature communications · 2025Article
- Bridging the gaps: advanced techniques to unlock lipid function in plant reproductive development.Plant reproduction · 2025Review
- Transcriptional and metabolic analysis of oleic acid synthesis in seedless and tenera oil palm species.Frontiers in plant science · 2025Article
- BnLPAT2 gene regulates oil accumulation in Brassica napus by modulating linoleic and linolenic acid levels in seeds.PloS one · 2025Article
- Transcriptome-referenced association study provides insights into the regulation of oil and fatty acid biosynthesis in Torreya grandis kernel.Journal of advanced research · 2024Article
- Article
- Evolutionary origin and gradual accumulation with plant evolution of the LACS family.BMC plant biology · 2024Article
- 3D genome structural variations play important roles in regulating seed oil content of Brassica napus.Plant communications · 2024Article
- Molecular Mapping of Putative Genomic Regions Controlling Fruit and Seed Morphology of Watermelon.International journal of molecular sciences · 2023Article
- Genome-Wide Analysis and Functional Characterization ofInternational journal of molecular sciences · 2023Article
- Cytological, transcriptome and miRNome temporal landscapes decode enhancement of rice grain size.BMC biology · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame.Frontiers in plant science · 2023Article
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Longer Duration of Active Oil Biosynthesis during Seed Development Is Crucial for High Oil Yield-Lessons from Genome-Wide In Silico Mining and RNA-Seq Validation in Sesame.Plants (Basel, Switzerland) · 2022Article
- Genome-Wide Identification and Expression Profiling Analysis of the Long-Chain Acyl-CoA Synthetases Reveal Their Potential Roles in Wheat Male Fertility.International journal of molecular sciences · 2022Article
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8 authors at 1 institution in 1 country.
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Abstract
backgroundTriacylglycerols (TAGs) are the main composition of plant seed oil. Long-chain acyl-coenzyme A synthetases (LACSs) catalyze the synthesis of long-chain acyl-coenzyme A, which is one of the primary substrates for TAG synthesis. In Arabidopsis, the LACS gene family contains nine members, among which LACS1 and LACS9 have overlapping functions in TAG biosynthesis. However, functional characterization of LACS proteins in rapeseed have been rarely reported.
resultsAn orthologue of the Arabidopsis LACS2 gene (BnLACS2) that is highly expressed in developing seeds was identified in rapeseed (Brassica napus). The BnLACS2-GFP fusion protein was mainly localized to the endoplasmic reticulum, where TAG biosynthesis occurs. Interestingly, overexpression of the BnLACS2 gene resulted in significantly higher oil contents in transgenic rapeseed plants compared to wild type, while BnLACS2-RNAi transgenic rapeseed plants had decreased oil contents. Furthermore, quantitative real-time PCR expression data revealed that the expression of several genes involved in glycolysis, as well as fatty acid (FA) and lipid biosynthesis, was also affected in transgenic plants.
conclusionsA long chain acyl-CoA synthetase, BnLACS2, located in the endoplasmic reticulum was identified in B. napus. Overexpression of BnLACS2 in yeast and rapeseed could increase oil content, while BnLACS2-RNAi transgenic rapeseed plants exhibited decreased oil content. Furthermore, BnLACS2 transcription increased the expression of genes involved in glycolysis, and FA and lipid synthesis in developing seeds. These results suggested that BnLACS2 is an important factor for seed oil production in B. napus.
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