ArticleStem cell research2020
Isolation of muscle stem cells from rat skeletal muscles.
Article in Stem cell research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- MuSC cultivation for cultured meat production: recent advances, current limitations, and future directions.Food science of animal resources · 2026Review
- An Optimized Pre-Plating Method for the Isolation and Culture of Human Primary Satellite Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Stem Cells Associated with Adult Skeletal Muscle Can Form Beating Cardiac Tissue In Vitro in Response to Media Containing Heparin, Dexamethasone, Growth Factors and Hydrogen Peroxide.International journal of molecular sciences · 2025Article
- Effects of Release of TSG-6 from Heparin Hydrogels on Supraspinatus Muscle Regeneration.Tissue engineering. Part A · 2025Article
- Satellite myoblast and mesenchymal stem cell injections decrease fatty degeneration after rotator cuff tear in rats.Journal of experimental orthopaedics · 2024Article
- Exosomes Secreted During Myogenic Differentiation of Human Fetal Cartilage-Derived Progenitor Cells Promote Skeletal Muscle Regeneration through miR-145-5p.Tissue engineering and regenerative medicine · 2024Article
- Methods to Isolate Muscle Stem Cells for Cell-Based Cultured Meat Production: A Review.Animals : an open access journal from MDPI · 2024Review
- Protective effect of Luffa cylindrica Roemer against dexamethasone-induced muscle atrophy in primary rat skeletal muscle cells.Journal of muscle research and cell motility · 2024Article
- Muscle stem cells and fibro-adipogenic progenitors in female pelvic floor muscle regeneration following birth injury.NPJ Regenerative medicine · 2022Article
- In Vitro Culturing of Adult Stem Cells: The Importance of Serum and Atmospheric Oxygen.Advances in experimental medicine and biology · 2022Review
- Quantifying the Effects of Aging on Morphological and Cellular Properties of Human Female Pelvic Floor Muscles.Annals of biomedical engineering · 2021Article
- The Expression Profiles of mRNAs and lncRNAs in Buffalo Muscle Stem Cells Driving Myogenic Differentiation.Frontiers in genetics · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Muscle stem cells (MuSCs) are involved in homeostatic maintenance of skeletal muscle and play a central role in muscle regeneration in response to injury. Thus, understanding MuSC autonomous properties is of fundamental importance for studies of muscle degenerative diseases and muscle plasticity. Rat, as an animal model, has been widely used in the skeletal muscle field, however rat MuSC isolation through fluorescence-activated cell sorting has never been described. This work validates a protocol for effective MuSC isolation from rat skeletal muscles. Tibialis anterior was harvested from female rats and digested for isolation of MuSCs. Three protocols, employing different cell surface markers (CD106, CD56, and CD29), were compared for their ability to isolate a highly enriched MuSC population. Cells isolated using only CD106 as a positive marker showed high expression of Pax7, ability to progress through myogenic lineage while in culture, and complete differentiation in serum-deprived conditions. The protocol was further validated in gastrocnemius, diaphragm, and the individual components of the pelvic floor muscle complex (coccygeus, iliocaudalis, and pubocaudalis), proving to be reproducible. CD106 is an efficient marker for reliable isolation of MuSCs from a variety of rat skeletal muscles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.