ArticlePlant biotechnology journal2020
Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 81 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Molecular Mechanisms and Regulation of Anthocyanin Biosynthesis in Rosaceae Fruits: A Review.Food science & nutrition · 2026Review
- Anthocyanin accumulation in fruit crops: a multi-layered regulatory network integrating molecular, epigenetic, hormonal and environmental regulation.Molecular biology reports · 2026Review
- Transcription factor MdMYB6L represses anthocyanin biosynthesis of apple skin at low-altitude regions.Plant cell reports · 2026Article
- TheFood chemistry. Molecular sciences · 2026Article
- Allele-specific methylation, and InDels ofHorticulture research · 2026Article
- Combined Transcriptome and Metabolome Analysis of the Quality Change Mechanism of the 'Pingguoli' Pear with a Large-Fruited Bud Mutation.Plants (Basel, Switzerland) · 2026Article
- Article
- Identification of DNA Methyltransferase/Demethylase Genes and 5-Azacytidine's Impact on β-Elemene and Methylation inBiology · 2025Article
- The grape berry methylome reveals tissue-specific features associated with metabolism in ripening.Horticulture research · 2025Article
- Genome-Wide Identification and Expression Profiling of the RNA-Directed DNA Methylation Pathway Genes inPlants (Basel, Switzerland) · 2025Article
- CHH demethylation in the ZmGST2 promoter enhances maize drought tolerance by regulating ROS scavenging and root growth.BMC plant biology · 2025Article
- C2H2-type zinc finger protein transcription factor MdZAT1 plays a negative role in anthocyanin biosynthesis in apple.Molecular horticulture · 2025Article
- Combined analysis of lncRNAs and mRNAs associated with coloration and wax formation during 'Fumei' Apple development.BMC plant biology · 2025Article
- Identification of key genes controlling anthocyanin biosynthesis in the fruits of a bud variety of Tarocco blood-orange.BMC plant biology · 2025Article
- Review
- CHH hypermethylation contributes to the early ripening of grapes revealed by DNA methylome landscape of 'Kyoho' and its bud mutant.Horticulture research · 2025Article
- Genome sequencing of 'Fuji' apple clonal varieties reveals genetic mechanism of the spur-type morphology.Nature communications · 2024Article
- Transcription factors LvBBX24 and LvbZIP44 coordinated anthocyanin accumulation in response to light in lily petals.Horticulture research · 2024Article
- CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum.Planta · 2024Article
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Authors and funding
16 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylation at the MdMYB1 promoter in apple sports has been reported as a regulator of the anthocyanin pathway, but little is known about how the locus is recognized by the methylation machinery to regulate anthocyanin accumulation. In this study, we analysed three differently coloured 'Fuji' apples and found that differences in the transcript levels of MdMYB1, which encodes a key regulator of anthocyanin biosynthesis, control the anthocyanin content (and therefore colour) in fruit skin. The CHH methylation levels in the MR3 region (-1246 to -780) of the MdMYB1 promoter were found to be negatively correlated with MdMYB1 expression. Thus, they were ideal materials to study DNA methylation in apple sports. The protein of RNA-directed DNA methylation (RdDM) pathway responsible for CHH methylation, MdAGO4, was found to interact with the MdMYB1 promoter. MdAGO4s can interact with MdRDM1 and MdDRM2s to form an effector complex, fulfilling CHH methylation. When MdAGO4s and MdDRM2s were overexpressed in apple calli and Arabidopsis mutants, those proteins increase the CHH methylation of AGO4-binding sites. In electrophoretic mobility shift assays, MdAGO4s were found to specifically bind to sequence containing ATATCAGA. Knockdown of MdNRPE1 did not affect the binding of MdAGO4s to the c3 region of the MdMYB1 promoter in 35S::AGO4 calli. Taken together, our data show that the MdMYB1 locus is methylated through binding of MdAGO4s to the MdMYB1 promoter to regulate anthocyanin biosynthesis by the RdDM pathway.
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