ArticlePloS one2020
A bushel of viruses: Identification of seventeen novel putative viruses by RNA-seq in six apple trees.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Prevalence, Molecular Detection, andViruses · 2026Article
- Haplotype-phased 'Ottawa 3' genome unravels differential reaction of apple rootstock roots to mixed viral infection.The Plant journal : for cell and molecular biology · 2026Article
- Characterising the Associated Virome and Microbiota of Asian Citrus Psyllid (Pathogens (Basel, Switzerland) · 2025Article
- Afrina barna-like Virus, a Novel Virus Associated withViruses · 2025Article
- Comprehensive Virome Profiling of Apple Mosaic Disease-Affected Trees in Iran Using RT-PCR and Next-Generation Sequencing.Viruses · 2025Article
- High-Throughput Sequencing Reveals Apple Virome Diversity and Novel Viruses in the Czech Republic.Viruses · 2025Article
- Discovery and characterization of a novel carlavirus in Ligularia jaluensis plants.Virologica Sinica · 2025Article
- Unveiling viral threats to temperate pome fruits: characterization, transmission, and sustainable management strategies.Frontiers in microbiology · 2025Review
- Apple varieties, diseases, and distinguishing between fresh and rotten through deep learning approaches.PloS one · 2025Article
- Viral Diversity in Mixed Tree Fruit Production Systems Determined through Bee-Mediated Pollen Collection.Viruses · 2024Article
- Investigating the Role of Viruses in the Rapid Decline of Young Apple Trees in High-Density Orchards in New York.Plants (Basel, Switzerland) · 2024Article
- Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries.The plant pathology journal · 2024Article
- Article
- Plant virus diversity in bee and pollen samples from apple (Frontiers in plant science · 2024Article
- PhytoPipe: a phytosanitary pipeline for plant pathogen detection and diagnosis using RNA-seq data.BMC bioinformatics · 2023Article
- The Rapid Apple Decline Phenomenon: Current Status and Expected Associated Factors in Korea.The plant pathology journal · 2023Review
- Clarithromycin Suppresses Apple Proliferation Phytoplasma in Explant Cultures.Plants (Basel, Switzerland) · 2023Article
- Complete Genome Characterization ofPlants (Basel, Switzerland) · 2023Article
- Article
- The Comparative Root System Architecture of Declining and Non-Declining Trees in Two Apple Orchards in New York.Plants (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apple decline in Washington state has been increasing in incidence, particularly on Honeycrisp trees grown on G.935 rootstock. In this disease the trees exhibit dieback with necrosis at the graft union and in the rootstock. The cause of this disease remains unknown. To identify viral candidates, RNA-seq was performed on six trees: four trees exhibiting decline and two healthy trees. Across the samples, eight known viruses and Apple hammerhead viroid were detected, however none appear to be specifically associated with the disease. A BLASTx analysis of the RNA-seq data was performed to identify novel viruses that might be associated with apple decline. Seventeen novel putative viruses were detected, including an ilarvirus, two tombus-like viruses, a barna-like virus, a picorna-like virus, three ourmia-like viruses, three partiti-like viruses, and two narna-like viruses. Four additional viruses could not be classified. Three of the viruses appeared to be missing key genes, suggesting they may be dependent upon helper viruses for their function. Others showed a specific tropism, being detected only in the roots or only in the leaves. While, like the known apple viruses, none were consistently associated with diseased trees, it is possible these viruses may have a synergistic effect when co-infecting that could contribute to disease. Or the presence of these viruses may weaken the trees for some other factor that ultimately causes decline. Additional research will be needed to determine how these novel viruses contribute to apple decline.
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