Evidence map›Paper›PMID 31927905›Full record

ArticleACS applied materials & interfaces2020

An Innovative Nanobody-Based Electrochemical Immunosensor Using Decorated Nylon Nanofibers for Point-of-Care Monitoring of Human Exposure to Pyrethroid Insecticides.

Ahmed Y El-Moghazy, Jingqian Huo, Noha Amaly, Natalia Vasylieva, Bruce D Hammock, Gang Sun

Abstract read
In one paragraph

Article in ACS applied materials & interfaces, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
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  3. Detection of AFBFoods (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ahmed Y El-MoghazyDepartment of Biological and Agricultural Engineering , University of California , Davis , California 95616 , USA.ORCID http://orcid.org/0000-0002-5743-7305
Jingqian HuoDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center , University of California , Davis , California 95616 , United States.
Noha AmalyDepartment of Biological and Agricultural Engineering , University of California , Davis , California 95616 , USA.
Natalia VasylievaDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center , University of California , Davis , California 95616 , United States.
Bruce D HammockDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center , University of California , Davis , California 95616 , United States.ORCID http://orcid.org/0000-0003-1408-8317
Gang SunDepartment of Biological and Agricultural Engineering , University of California , Davis , California 95616 , USA.ORCID http://orcid.org/0000-0002-6608-9971

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
NIEHS NIH HHS P42 ES004699
6 · The paper itself

Abstract

A novel ultrasensitive nanobody-based electrochemical immunoassay was prepared for assessing human exposure to pyrethroid insecticides. 3-Phenoxybenzoic acid (3-PBA) is a common human urinary metabolite for numerous pyrethroids, which broadly served as a biomarker for following the human exposure to this pesticide group. The 3-PBA detection was via a direct competition for binding to alkaline phosphatase-embedded nanobodies between free 3-PBA and a 3-PBA-bovine serum albumin conjugate covalently immobilized onto citric acid-decorated nylon nanofibers, which were incorporated on a screen-printed electrode (SPE). Electrochemical impedance spectroscopy (EIS) was utilized to support the advantage of the employment of nanofibrous membranes and the success of the immunosensor assembly. The coupling between the nanofiber and nanobody technologies provided an ultrasensitive and selective immunosensor for 3-PBA detection in the range of 0.8 to 1000 pg mL

Indexed as

BenzoatesElectrochemical TechniquesEnvironmental ExposureHumansImmunoassayLimit of DetectionNanofibersNylonsPoint-of-Care SystemsPyrethrinsReproducibility of ResultsSingle-Domain Antibodies3-phenoxybenzoic acidBenzoatesNylonsPyrethrinsSingle-Domain Antibodies3-PBAelectrochemical immunosensornanobodynanofibersnylonpoint-of-carepyrethroids

Identifiers

PMID31927905
PMCPMC7799635

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.