Evidence map›Paper›PMID 31927667›Full record

ArticleMikrochimica acta2020

Rolling circle amplification based colorimetric determination of Staphylococcus aureus.

Yanan Li, Junying Wang, Shuo Wang, Junping Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

  1. Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
  2. Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 1 country.

Yanan LiState Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin, 300457, People's Republic of China.
Junying WangChinese Academy of Agricultural Sciences, Biotechnology Research Institute, Haidian District, Beijing, 010010, People's Republic of China.
Shuo WangMedical college, Nankai University, No.38 Tongyan Road, Jinnan District, Tianjin, 300350, People's Republic of China. swang@tust.edu.cn.
Junping WangState Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin, 300457, People's Republic of China. wangjp@tust.edu.cn.
Biotechnology Research Institute · CNChinese Academy of Agricultural Sciences · CNNankai University · CNTianjin University of Science and Technology · CN

Funding

Special Project of Tianjin Innovation Platform 17PTGCCX00230The National Key R & D Program of China 2016YFD0401202Tianjin science and technology planning project 18PTSYJC00130
6 · The paper itself

Abstract

A colorimetric microplate assay for determination of Staphylococcus aureus DNA is described. Linear padlock probes were designed to recognize target sequences. After DNA binding, the linear padlock probes were circularized by ligation and then hybridize with biotin-labeled capture probes. Biotin-labeled capture probes act as primers to initiate the RCA. The biotin-labeled RCA products hybridize with digoxin-labeled signal probes fixed on streptavidin-functionalized wells of a 96-well plate. To enhance sensitivity, an AuNP-anti-digoxigenin-POx-HRP conjugate was added to the wells and then bound to digoxin-labeled signalling probes. The oxidation of tetramethylbenzidine (TMB) by H

Indexed as

AnimalsArmoraciaBenzidinesChickensColorimetryColoring AgentsDNA, BacterialDNA ProbesFood ContaminationFood MicrobiologyLimit of DetectionMilkNucleic Acid Amplification TechniquesNucleic Acid HybridizationPoultryStaphylococcus aureus3,3',5,5'-tetramethylbenzidineBenzidinesColoring AgentsDNA, BacterialDNA ProbesBiotin–streptavidin systemColorimetric microplate assayMultifunctional gold nanoparticlesRolling circle amplification (RCA)Staphylococcus aureus

Identifiers

PMID31927667
OpenAlexW2999491568

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.