Evidence map›Paper›PMID 31927011›Full record

ArticleFitoterapia2020

Bufadienolides and anti-angiogenic homoisoflavonoids from Rhodocodon cryptopodus, Rhodocodon rotundus and Rhodocodon cyathiformis.

Hannah Whitmore, Kamakshi Sishtla, Walter Knirsch, Jacky L Andriantiana, Sianne Schwikkard, Eduard Mas-Claret, Sarah M Nassief, Sani M Isyaka, Timothy W Corson, Dulcie A Mulholland

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fitoterapia, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 5 countries.

Hannah WhitmoreNatural Products Research Group, Department of Chemistry, University of Surrey, Guildford GU2 7XH, United Kingdom.
Kamakshi SishtlaEugene and Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, 1160 W. Michigan St., Indianapolis, IN 46202, USA.
Walter KnirschInstitute of Plant Sciences, NAWI Graz, Karl-Franzens University Graz, Holteigasse 6, A-8010 Graz, Austria.
Jacky L AndriantianaParc Botanique et Zoologique de Tsimbazaza, Rue Fernand Kassanga, Antananarivo 101, Madagascar.
Sianne SchwikkardSchool of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston-upon-Thames KT1 2EE, United Kingdom.
Eduard Mas-ClaretNatural Products Research Group, Department of Chemistry, University of Surrey, Guildford GU2 7XH, United Kingdom.
Sarah M NassiefNatural Products Research Group, Department of Chemistry, University of Surrey, Guildford GU2 7XH, United Kingdom.
Sani M IsyakaNatural Products Research Group, Department of Chemistry, University of Surrey, Guildford GU2 7XH, United Kingdom; School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa.
Timothy W CorsonEugene and Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, 1160 W. Michigan St., Indianapolis, IN 46202, USA. Electronic address: tcorson@iu.edu.
Dulcie A MulhollandNatural Products Research Group, Department of Chemistry, University of Surrey, Guildford GU2 7XH, United Kingdom; School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa. Electronic address: d.mulholland@surrey.ac.uk.
University of Surrey · GBIndiana University – Purdue University Indianapolis · USKingston University · GBTsimbazaza Zoo · MGUniversity of Graz · ATUniversity of KwaZulu-Natal · ZA

Funding

Ferrochelatase as a mediator of ocular angiogenesisR01EY025641 · NEI · UNIVERSITY OF TORONTO · PI Timothy W Corson · 2016 to 2026
$3.5M
NEI NIH HHS R01 EY025641
6 · The paper itself

Abstract

backgroundHomoisoflavonoids have been shown to have potent anti-proliferative activities in endothelial cells over other cell types and have demonstrated a strong antiangiogenic potential in vitro and in vivo in animal models of ocular neovascularization. Three species of Rhodocodon (Scilloideaea subfamily of the Asparagaceae family), endemic to Madagascar, R. cryptopodus, R. rotundus and R. cyathiformis, were investigated. PURPOSE: To isolate and test homoisoflavonoids for their antiangiogenic activity against human retinal microvascular endothelial cells (HRECs), as well as specificity against other ocular cell lines.

methodsPlant material was extracted at room temperature with EtOH. Compounds were isolated using flash column chromatography and were identified using NMR and CD spectroscopy and HRESIMS. Compounds were tested for antiproliferative effects on primary human microvascular retinal endothelial cells (HRECs), ARPE19 retinal pigment epithelial cells, 92-1 uveal melanoma cells, and Y79 retinoblastoma cells. HRECs exposed to compounds were also tested for migration and tube formation ability.

resultsTwo homoisoflavonoids, 3S-5,7-dihydroxy-(3'-hydroxy-4'-methoxybenzyl)-4-chromanone (1) and 3S-5,7-dihydroxy-(4'-hydroxy-3'-methoxybenzyl)-4-chromanone (2), were isolated along with four bufadienolides. Compound 1 was found to be non-specifically antiproliferative, with GI

conclusionSelect homoisoflavonoids have promise as antiangiogenic agents that are not generally cytotoxic.

Indexed as

AsparagaceaeBufanolidesCell MovementCell ProliferationEndothelial CellsHumansIsoflavonesNeovascularization, PhysiologicRetinal VesselsBufanolidesIsoflavonesAnti-angiogenicBufadienolidesEndothelial cellsHomoisoflavonoidsNeovascularizationRhodocodon

Identifiers

PMID31927011
PMCPMC7065379
OpenAlexW2999299303

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.