ArticleScientific reports2020
Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23).
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Fatty acid synthesis and utilization in gram-positive bacteria: insights fromMicrobiology and molecular biology reviews : MMBR · 2025Review
- A novel infective endocarditis virulence factor related to multiple functions for bacterial survival in blood was discovered inbioRxiv : the preprint server for biology · 2024Article
- Transcript Profiling of Nitroxoline-Treated Biofilms Shows Rapid Up-regulation of Iron Acquisition Gene Clusters.ACS infectious diseases · 2022Article
- Destination and Specific Impact of Different Bile Acids in the Intestinal PathogenFrontiers in microbiology · 2022Article
- Article
- Mini Review: Bacterial Membrane Composition and Its Modulation in Response to Stress.Frontiers in molecular biosciences · 2021Review
- Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors inAntibiotics (Basel, Switzerland) · 2020Article
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Authors and funding
7 authors.
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Abstract
Fluorescence-tags, commonly used to visualize the spatial distribution of proteins within cells, can influence the localization of the tagged proteins by affecting their stability, interaction with other proteins or the induction of oligomerization artifacts. To circumvent these obstacles, a protocol was developed to generate 50 nm thick serial sections suitable for immunogold labeling and subsequent reconstruction of the spatial distribution of immuno-labeled native proteins within individual bacterial cells. Applying this method, we show a cellular distribution of the staphylococcal alkaline shock protein 23 (Asp23), which is compatible with filament formation, a property of Asp23 that we also demonstrate in vitro.
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