ArticleScientific reports2020
Label-free impedance flow cytometry for nanotoxicity screening.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Structural, Compositional, and Dielectric State Profiling in Label-Free Single-Cell Monitoring.Small methods · 2026Review
- Biocompatibility testing and antioxidant properties of cerium dioxide nanoparticles in human nervous system cells.Archives of toxicology · 2025Article
- Noninvasive real-time monitoring of cellular spatiotemporal dynamics via machine learning-enhanced electrical impedance spectroscopy.Science advances · 2025Article
- A template wizard for the cocreation of machine-readable data-reporting to harmonize the evaluation of (nano)materials.Nature protocols · 2024Review
- Review
- Bio-inspired microfluidics: A review.Biomicrofluidics · 2023Review
- Using Impedance Flow Cytometry for Rapid Viability Classification of Heat-Treated Bacteria.ACS omega · 2023Article
- A Simple Micromilled Microfluidic Impedance Cytometer with Vertical Parallel Electrodes for Cell Viability Analysis.Micromachines · 2023Article
- Label-Free Microfluidic Impedance Cytometry for Acrosome Integrity Assessment of Boar Spermatozoa.Biosensors · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- A Review on Conventional and Advanced Methods for Nanotoxicology Evaluation of Engineered Nanomaterials.Molecules (Basel, Switzerland) · 2021Review
- Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate.Pharmaceutics · 2021Review
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- Engineered Nanomaterials: The Challenges and Opportunities for Nanomedicines.International journal of nanomedicine · 2021Review
- Epigenetics in Breast Cancer Therapy-New Strategies and Future Nanomedicine Perspectives.Cancers · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label-free impedance flow cytometry (IFC) for in vitro nanotoxicity screening, which avoids time-consuming labelling steps and minimizes possible NM-induced interferences. U937 human lymphoma cells were exposed for 24 h to eight different nanomaterials at five concentrations (2, 10, 20, 50, and 100 μg/mL). The NMs' effect on viability was measured using IFC and the results were compared to those obtained by trypan blue (TB) dye exclusion and conventional flow cytometry (FC). To discriminate viable from necrotic cells, the IFC measurement settings regarding signal trigger level and frequency, as well as the buffer composition, were optimised. A clear discrimination between viable and necrotic cells was obtained at 6 MHz in a sucrose-based measurement buffer. Nanomaterial-induced interferences were not detected for IFC. The IFC and TB assay results were in accordance for all NMs. The IFC was found to be robust, reliable and less prone to interferences due to the advantage of being label-free.
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Registered trials
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