ArticleScientific reports2020
DNA extraction approaches substantially influence the assessment of the human breast milk microbiome.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 91 citations in OpenAlex.
- The human breast milk microbiome: a landscape review of its composition, origins, and impact on infant health.Archives of microbiology · 2026Review
- Enhancing DNA recovery in low-biomass snow algae samples: a comparative study of extraction methods and their effect on community composition.Applied and environmental microbiology · 2026Article
- Ig-microbiota binding patterns in mothers and infants: a scoping review.Gut microbiome (Cambridge, England) · 2026Review
- Anellovirus constraint from 2 to 6 months postpartum followed by betatorquevirus and gammatorquevirus dominance in serum and milk.Journal of virology · 2025Article
- Development of a Rapid, Culture-Free, Universal Microbial Identification System Using Internal Transcribed Spacer Targeting Primers.The Journal of infectious diseases · 2025Article
- Optimization ofJournal of environmental engineering (New York, N.Y.) · 2025Article
- Human Milk Microbiome from Polish Women Giving Birth via Vaginal Delivery-Pilot Study.Biology · 2025Article
- De-biasing microbiome sequencing data: bacterial morphology-based correction of extraction bias and correlates of chimera formation.Microbiome · 2025Article
- The impact of breastfeeding on the preterm infant's microbiome and metabolome: a pilot study.Pediatric research · 2025Observational
- Comparative pilot study of three commercial kits for bacterial DNA extraction from human subgingival biofilm samples collected with a single paper point.Journal of oral microbiology · 2025Article
- Low Bacterial Biomass in Human Pancreatic Cancer and Adjacent Normal Tissue.International journal of molecular sciences · 2024Article
- Metrological evaluation of DNA extraction method effects on the bacterial microbiome and resistome in sputum.mSystems · 2024Article
- The impact of DNA extraction on the quantification ofMicrobiology spectrum · 2024Article
- Impact of intrapartum antibiotic prophylaxis on the oral and fecal bacteriomes of children in the first week of life.Scientific reports · 2024Article
- Residents or Tourists: Is the Lactating Mammary Gland Colonized by Residential Microbiota?Microorganisms · 2024Review
- Microbiome: Mammalian milk microbiomes: sources of diversity, potential functions, and future research directions.Reproduction & fertility · 2024Review
- Development and validation of a PMA-qPCR method for accurate quantification of viableFrontiers in microbiology · 2024Article
- Utility of dairy microbiome as a tool for authentication and traceability.Open life sciences · 2024Review
- Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques.ISME communications · 2023Article
- COVID-19 mRNA vaccine-mediated antibodies in human breast milk and their association with breast milk microbiota composition.NPJ vaccines · 2023Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant. However, the composition of this bacterial community differs considerably between studies. We hypothesised that bacterial DNA extraction methodology from breast milk samples are a substantial contributor to these inter-study differences. We tested this hypothesis by applying five widely employed methodologies to a mock breast milk sample and four individual human breast milk samples. Significant differences in DNA yield and purity were observed between methods (P < 0.05). Microbiota composition, assessed by 16S rRNA gene amplicon sequencing, also differed significantly with extraction methodology (P < 0.05), including in the contribution of contaminant signal. Concerningly, many of the bacterial taxa identified here as contaminants have been reported as components of the breast milk microbiome in other studies. These findings highlight the importance of using stringent, well-validated, DNA extraction methodologies for analysis of the breast milk microbiome, and exercising caution interpreting microbiota data from low-biomass contexts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.