Evidence map›Paper›PMID 31923446›Full record

ArticleJournal of virological methods2020

Field-based method for assessing duration of infectivity for influenza A viruses in the environment.

Andrew B Reeves, Andrew M Ramey, Joshua C Koch, Rebecca L Poulson, David E Stallknecht

Open access · greenAbstract read
In one paragraph

Article in Journal of virological methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Andrew B ReevesU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, Alaska 99508, USA. Electronic address: areeves@usgs.gov.
Andrew M RameyU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, Alaska 99508, USA.
Joshua C KochU.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, Alaska 99508, USA.
Rebecca L PoulsonSoutheastern Cooperative Wildlife Disease Study, 589 D. W. Brooks Drive, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
David E StallknechtSoutheastern Cooperative Wildlife Disease Study, 589 D. W. Brooks Drive, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
University of Georgia · USU.S. Geological Survey, Alaska Science CenterUnited States Geological Survey · US

Funding

NIAID NIH HHS HHSN272201400006C
6 · The paper itself

Abstract

Understanding influenza A virus (IAV) persistence in wetlands is limited by a paucity of field studies relating to the maintenance of infectivity over time. The duration of IAV infectivity in water has been assessed under variable laboratory conditions, but results are difficult to translate to more complex field conditions. We tested a field-based method to assess the viability of IAVs in an Alaska wetland during fall and winter which incorporated physical and chemical properties of the waterbody in which samples were held. Filtered pond water was inoculated with avian fecal samples collected from the environment, aliquoted into a series of duplicate sealed vials and submerged back in the wetland for up to 132 days (October 2018-March 2019). Sample aliquots were sequentially recovered and tested for IAVs by rRT-PCR and virus isolation. One sample remained rRT-PCR positive for the duration of the study and virus isolation positive for 118 days. The surrounding water temperature was 1°-6 °C with near neutral pH (6.6-7.3) for the duration of the study. This proof of concept study demonstrates a protocol for testing the persistence of infectious IAV naturally shed from waterfowl under ambient environmental conditions.

Indexed as

Microbial ViabilityTemperatureAlaskaAnimalsAnimals, WildBirdsFecesHydrogen-Ion ConcentrationInfluenza A virusInfluenza in BirdsPondsProof of Concept StudyRNA, ViralVirologyVirus SheddingRNA, ViralAlaskaInfluenza A virusViabilityWaterfowlWetland

Identifiers

PMID31923446
PMCPMC9289940
OpenAlexW2999633922

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.