Evidence map›Paper›PMID 31923252›Full record

ArticlePloS one2020

Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit.

A M Abdelatty, O A M Badr, S A Mohamed, M S Khattab, Sh M Dessouki, O A A Farid, A A Elolimy, O G Sakr, M A Elhady, G Mehesen and 1 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
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  4. Review
  5. Food science & nutrition · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

A M AbdelattyDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.ORCID 0000-0002-6544-5145
O A M BadrDepartment of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt.ORCID 0000-0002-2318-9630
S A MohamedDepartment of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt.
M S KhattabDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Sh M DessoukiDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
O A A FaridDepartment of Physiology, National Organization for Drug Control and Research, Giza, Egypt.
A A ElolimyDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.
O G SakrDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
M A ElhadyDepartment of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
G MehesenDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
M BionazDepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR, United States of America.
Cairo University · EGBenha University · EGNational Organization for Drug Control and Research · EGNational Research Centre · EGOregon State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conjugated linoleic acid (CLA) is known for its multiple benefits including improvement of growth, increasing lean mass, and anti-carcinogenic effects. However, when used in long-term supplementations CLA does not improve semen parameters in boar and bull and reduces fertility in Japanese quails. The content of unsaturated fatty acids in dietary lipids plays a significant role in spermatogenesis owning the high proportion of unsaturated fatty acids in plasma membrane of sperms. Whether CLA plays a role in testicular tissue and epididymal fat is still unknown. Therefore, in this study we hypothesize that long-term supplementation of equal proportion of CLA isomer mix (c9,t11-CLA and t10,c12- CLA) in rabbit bucks might alter male reproductive potentials. Twelve V-Line weaned male rabbits were used in 26 weeks trial, rabbits were individually raised and randomly allocated into three dietary groups. Control group (CON) received a basal diet, a group received 0.5% CLA (CLA 0.5%), and a group received 1% CLA (CLA 1%). Rabbits were euthanized at the end of the trial and several parameters were evaluated related to growth, semen quality, and testicular and epididymal tissue histopathology and transcriptome. The long-term supplementation of CLA increased feed intake by 5% and body weight by 2-3%. CLA 1% decreased sperm progressive motility. In testicular tissue L-carnitine and α-tocopherol were decreased by CLA supplementation. In epididymal fat, CLA tended to decrease concentration of polyunsaturated fatty acids, the expression of SCD5 gene was upregulated by CLA 1% and CASP3 gene was upregulated by CLA 0.5%. Transcription of PPARG was downregulated by CLA. Feeding 1% CLA also decreased testicular epithelial thickness. Long-term supplementation of CLA modestly enhanced male rabbit growth, but negatively impacted male reproduction, especially at high dose of CLA.

Indexed as

ApoptosisLinoleic Acids, ConjugatedSpermatozoaSperm MotilityAnimalsBody WeightCarnitineDietary SupplementsDown-RegulationEpididymisFatty Acids, UnsaturatedMalePPAR gammaRabbitsSemen AnalysisStearoyl-CoA DesaturaseCarnitineFatty Acids, UnsaturatedLinoleic Acids, ConjugatedPPAR gammaStearoyl-CoA DesaturaseTestosterone

Identifiers

PMID31923252
PMCPMC6953797
OpenAlexW2999817713

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.