ArticlePLoS genetics2020
Mutations on ent-kaurene oxidase 1 encoding gene attenuate its enzyme activity of catalyzing the reaction from ent-kaurene to ent-kaurenoic acid and lead to delayed germination in rice.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- CRISPR/Cas9-Mediated Mutagenesis of OsERF94 Enhances Pre-Harvest Sprouting in Rice.Rice (New York, N.Y.) · 2026Article
- Engineering a Gibberellin-Switchable Dual-Use Line Based on Ent-Kaurene Oxidase Gene ZmKO1 Enables Two-Line Hybrid Seed Production in Maize.Plant biotechnology journal · 2026Article
- Comparative transcriptome analysis provides insights into the dwarfing mechanism of pear trees.BMC genomic data · 2026Article
- OsSAE1 orchestrates the antagonistical regulation of gibberellin and abscisic acid signaling to control rice seed germination.Journal of integrative plant biology · 2026Article
- Article
- Genome-Wide Identification of the RhoGAP Gene Family and Main Function of OsRhoGAP2 in Seed Germination of Rice by Transcriptome Analysis.Rice (New York, N.Y.) · 2025Article
- A MYB61-SWB9-KOs module regulates grain chalkiness via gibberellin biosynthesis in rice endosperm.Plant biotechnology journal · 2025Article
- Gibberellins: extending the Green Revolution.Journal of experimental botany · 2025Review
- The Mining for Flowering-Related Genes Based on De Novo Transcriptome Sequencing in the Endangered PlantInternational journal of molecular sciences · 2025Article
- Article
- Full-length transcriptome profiling ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Catalase associated with antagonistic changes of abscisic acid and gibberellin response, biosynthesis and catabolism is involved in eugenol-inhibited seed germination in rice.Plant cell reports · 2023Article
- Understanding of Hormonal Regulation in Rice Seed Germination.Life (Basel, Switzerland) · 2022Review
- Brassinosteroids regulate rice seed germination through the BZR1-RAmy3D transcriptional module.Plant physiology · 2022Article
- The Brassica napus fatty acid exporter FAX1-1 contributes to biological yield, seed oil content, and oil quality.Biotechnology for biofuels · 2021Article
- Genomic divergence during feralization reveals both conserved and distinct mechanisms of parallel weediness evolution.Communications biology · 2021Article
- OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling.Frontiers in plant science · 2021Article
- The Current Status of Research on Gibberellin Biosynthesis.Plant & cell physiology · 2020Review
- The Candidate Genes Underlying a Stably Expressed QTL for Low Temperature Germinability in Rice (Oryza sativa L.).Rice (New York, N.Y.) · 2020Article
- Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review.Plants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice seed germination is a critical step that determines its entire life circle, with seeds failing to germinate or pre-harvest sprouting both reduce grain yield. Nevertheless, the mechanisms underlying this complex biological event remain unclear. Previously, gibberellin has been shown to promote seed germination. In this study, a delayed seed germination rice mutant was obtained through screening of the EMS induced mutants. Besides of delayed germination, it also shows semi-dwarfism phenotype, which could be recovered by exogenous GA. Through re-sequencing on the mutant, wild-type and their F2 populations, we identified two continuous mutated sites on ent-kaurene oxidase 1 (OsKO1) gene, which result in the conversion from Thr to Met in the cytochrome P450 domain. Genetic complementary analysis and enzyme assay verified that the mutations in OsKO1 gene block the biosynthesis of GA and result in the defect phenotypes. Further analyses proved that OsKO1 could catalyze the reaction from ent-kaurene into ent-kaurenoic acid in GA biosynthesis mainly at seed germination and seedling stages, and the mutations decrease its activity to catalyze the step from ent-kaurenol to ent-kaurenoic acid in this reaction. Transcriptomic and proteomic data indicate that the defect on GA biosynthesis decreases its ability to mobilize starch and attenuate ABA signaling, therefore delay the germination process. The results provide some new insights into both GA biosynthesis and seed germination regulatory pathway in rice.
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