ArticleAmerican journal of human biology : the official journal of the Human Biology Council2020
Dried blood spots: Effects of less than optimal collection, shipping time, heat, and humidity.
Article in American journal of human biology : the official journal of the Human Biology Council, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Results of the AiM, PLan, and act on LIFestYles (AMPLIFY) Weight Loss Intervention Among Survivors of Obesity-Related Cancers: A Randomized Clinical Trial.Journal of the National Comprehensive Cancer Network : JNCCN · 2026Trial
- A randomised study of nurse collected venous blood and self-collected dried blood spots for the assessment of cardiovascular risk factors in the Understanding Society Innovation Panel.Scientific reports · 2023Trial
- New Perspectives of Multiplex Mass Spectrometry Blood Protein Quantification on Microsamples in Biological Monitoring of Elderly Patients.International journal of molecular sciences · 2023Trial
- An Investigation of Instability in Dried Blood Spot Samples for Pharmacokinetic Sampling in Phase 3 Trials of Verubecestat.The AAPS journal · 2022Trial
- From field prick to decision-grade proof: a dried blood spot and volumetric absorptive microsampling framework for verifying chemical-agent exposure.Forensic toxicology · 2026Article
- Sources and transmission dynamics ofmedRxiv : the preprint server for health sciences · 2026Article
- Newborn Screening in Saudi Arabia: Brief History, Current Practice, and Future Direction.International journal of neonatal screening · 2026Review
- The analytical potential of the dry blood spot technique for pesticide biomonitoring - a review.Environmental monitoring and assessment · 2025Review
- Investigating psychosocial factors and systemic inflammation using dried blood spots: a scoping review.Social psychiatry and psychiatric epidemiology · 2025Article
- Abnormal Blood Biomarkers and Cumulative Disability Burden in Middle-Aged and Older Adults: Evidence from Two Nationally Representative Surveys in the United States and China.Journal of cardiovascular development and disease · 2025Article
- Pre-analytic assessment of dried blood and dried plasma spots: integration in mass spectrometry-based metabolomics and lipidomics workflow.Analytical and bioanalytical chemistry · 2025Article
- Demographic and Clinical Factors Associated With SARS-CoV-2 Anti-Nucleocapsid Antibody Response Among Previously Infected US Adults: The C4R Study.Open forum infectious diseases · 2025Article
- Development and validation of the UHPLC-MS/MS method for the quantitative determination of 25 PFAS in dried blood spots.Analytical and bioanalytical chemistry · 2024Article
- The Impact of Delayed Processing of Chilled Whole Blood Specimens on the Measurement of Nutritional Biomarkers in the United Kingdom National Diet and Nutrition Survey Rolling Programme.The Journal of nutrition · 2024Article
- Sex differences in association of joint glycemic, blood pressure and lipid control and two-year risk of falls among older adults with diabetes.Journal of diabetes and its complications · 2024Article
- Remote dried blood spot collection for inflammatory markers in older adults is feasible, reliable, and valid.Brain, behavior, and immunity · 2024Article
- The development, validation and application of remote blood sample collection in telehealth programmes.Journal of telemedicine and telecare · 2024Article
- Harmonization of four biomarkers across nine nationally representative studies of older persons.American journal of human biology : the official journal of the Human Biology Council · 2024Article
- Dried blood spot based biomarkers in the Health and Retirement Study: 2006 to 2016.American journal of human biology : the official journal of the Human Biology Council · 2024Article
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Authors and funding
10 authors.
Funding
Abstract
objectivesThis study investigates how factors related to collection, storage, transport time, and environmental conditions affect the quality and accuracy of analyses of dried blood spot (DBS) samples.
methodsData come from the 2016 Health and Retirement Study (HRS) DBS laboratory reports and the HRS merged with the National Climatic Data Center (NCDC) Global Historical Climate Network Daily (NCDC GHCN-Daily) and the NCDC Local Climatological Data, by zip code. We ran regression models to examine the associations between assay values based on DBS for five analytes (total cholesterol, high-density lipoprotein (HDL) cholesterol, glycosylated hemoglobin (HbA1c), C-reactive protein (CRP), and cystatin C) and the characteristics of DBS cards and drops, shipping time, and temperature, and humidity at the time of collection.
resultsWe found cholesterol measures to be sensitive to many factors including small spots, shipping time, high temperature and humidity. Small spots in DBS cards are related to lower values across all analytes. Longer DBS transit time before freezing is associated with lower values of total and HDL cholesterol and cystatin C. Results were similar whether or not venous blood sample values were included in equations.
conclusionsSmall spots, long shipping time, and exposure to high temperature and humidity need to be avoided if possible. Quality of spots and cards and information on shipping time and conditions should be coded with the data to make adjustments in values when necessary. The different results across analytes indicate that results cannot be generalized to all DBS assays.
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