ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2020
Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone-like tissue formation.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- A noncanonical role for Jagged1 in endothelial mechanotransduction.The FEBS journal · 2026Article
- Strong living scaffolds for load-bearing musculoskeletal tissue regeneration.Materials today. Bio · 2025Review
- Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches.ACS biomaterials science & engineering · 2025Article
- Fully Defined 3D Hybrid System for Bone Tissue Engineering: Integration of MeHA-RGD/PCL-TCP Scaffolds With Human Stem Cells via 3D-Printed Vacuum-Assisted Cell Loading Device.Journal of tissue engineering and regenerative medicine · 2025Article
- Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds.Advanced healthcare materials · 2024Article
- Orbital shaking conditions augment human nasoseptal cartilage formation in 3D culture.Frontiers in bioengineering and biotechnology · 2024Article
- DONOR VARIABILITY IN HUMAN MESENCHYMAL STEM CELL OSTEOGENIC RESPONSE AS A FUNCTION OF PASSAGE CONDITIONS AND DONOR SEX.bioRxiv : the preprint server for biology · 2023Article
- The Impact of Culture Variables on a 3D Human In Vitro Bone Remodeling Model: A Design of Experiments Approach.Advanced healthcare materials · 2023Article
- Auxeticity as a Mechanobiological Tool to Create Meta-Biomaterials.ACS applied bio materials · 2023Review
- Effects of cell seeding technique and cell density on BMP-2 production in transduced human mesenchymal stem cells.Journal of biomedical materials research. Part A · 2022Article
- Sources, Selection, and Microenvironmental Preconditioning of Cells for Urethral Tissue Engineering.International journal of molecular sciences · 2022Review
- 3D Culture Modeling of Metastatic Breast Cancer Cells in Additive Manufactured Scaffolds.ACS applied materials & interfaces · 2022Article
- Article
- Optimization and Validation of a Custom-Designed Perfusion Bioreactor for Bone Tissue Engineering: Flow Assessment and Optimal Culture Environmental Conditions.Frontiers in bioengineering and biotechnology · 2022Article
- Orbital seeding of mesenchymal stromal cells increases osteogenic differentiation and bone-like tissue formation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Computational Characterization of The Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker.Micromachines · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In bone tissue engineering (TE), an efficient seeding and homogenous distribution of cells is needed to avoid cell loss and damage as well as to facilitate tissue development. Dynamic seeding methods seem to be superior to the static ones because they tend to result in a more homogeneous cell distribution by using kinetic forces. However, most dynamic seeding techniques are elaborate or require special equipment and its influence on the final bone tissue-engineered construct is not clear. In this study, we applied a simple, dynamic seeding method using an orbital shaker to seed human bone marrow-derived mesenchymal stromal cells (hBMSCs) on silk fibroin scaffolds. Significantly higher cell numbers with a more homogenous cell distribution, increased osteogenic differentiation, and mineral deposition were observed using the dynamic approach both for 4 and 6 hours as compared to the static seeding method. The positive influence of dynamic seeding could be attributed to both cell density and distribution but also nutrient supply during seeding and shear stresses (0.0-3.0 mPa) as determined by computational simulations. The influence of relevant mechanical stimuli during seeding should be investigated in the future, especially regarding the importance of mechanical cues for bone TE applications. Our results highlight the importance of adequate choice of seeding method and its impact on developing tissue-engineered constructs. The application of this simple seeding technique is not only recommended for bone TE but can also be used for seeding similar porous scaffolds with hBMSCs in other TE fields.
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