ArticleFrontiers in bioengineering and biotechnology2019
High-Throughput Screening of a 2-Keto-L-Gulonic Acid-Producing
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 19 citations in OpenAlex.
- Efficient Production of 2-Keto-l-Gulonic Acid via One-Step Fermentation UsingMicroorganisms · 2026Article
- Structural Insight into the Catalytic Mechanisms of an L-Sorbosone Dehydrogenase.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Identification of Gradient Promoters ofFrontiers in bioengineering and biotechnology · 2021Article
- Rapid Enabling ofFrontiers in bioengineering and biotechnology · 2021Article
- Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy.Micromachines · 2020Review
- High Throughput Screening Platform for a FAD-Dependent L-Sorbose Dehydrogenase.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput screening is a powerful tool for discovering strains in the natural environment that may be suitable for target production. Herein, a novel enzyme-based high-throughput screening method was developed for rapid screening of strains overproducing 2-keto-L-gulonic acid (2-KLG). The screening method detects changes in the fluorescence of reduced nicotinamide adenine dinucleotide (NADH) at 340 nm using a microplate reader when 2-KLG is degraded by 2-KLG reductase. In this research, three different 2-KLG reductases were expressed, purified, and studied. The 2-KLG reductase from
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Registered trials
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