Evidence map›Paper›PMID 31921407›Full record

ArticleCell & bioscience2020

Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier.

Bi Foua Claude Alain Gohi, Xue-Ying Liu, Hong-Yan Zeng, Sheng Xu, Kouassi Marius Honore Ake, Xiao-Ju Cao, Kai-Min Zou, Sheila Namulondo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Bi Foua Claude Alain Gohi1Biology and Chemical Engineering School, Panzhihua University, Panzhihua, 617000 Sichuan People's Republic of China.
Xue-Ying LiuEconomical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green, Zhuzhou, China.
Hong-Yan Zeng2Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan, 411105 Hunan People's Republic of China.ORCID 0000-0002-7892-2019
Sheng Xu2Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan, 411105 Hunan People's Republic of China.
Kouassi Marius Honore Ake5Faculty of Business Administration, Laval University, Pavillon Palasis-Prince, 2325 Rue de la Terrasse, G1V 0A6 Quebec City, Canada.
Xiao-Ju Cao2Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan, 411105 Hunan People's Republic of China.
Kai-Min Zou2Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan, 411105 Hunan People's Republic of China.
Sheila Namulondo6Institute of Comparative Literature and World Literature, College of Literature and Journalism, Xiangtan University, Xiangtan, 411105 Hunan People's Republic of China.
Hunan Institute of Engineering · CNHunan University of Technology · CNInstitute of World Literature of the Slovak Academy of Sciences · SKUniversité Laval · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A two-stage method of obtaining viable human amniotic stem cells (hAMSCs) in large-scale is described. First, human amniotic stem cells are isolated via dual enzyme (collagenase II and DNAase I) digestion. Next, relying on a culture of the cells from porous chitosan-based microspheres in vitro, high purity hAMSCs are obtained in large-scale. Dual enzymatic (collagenase II and DNase I) digestion provides a primary cell culture and first subculture with a lower contamination rate, higher purity and a larger number of isolated cells. The obtained hAMSCs were seeded onto chitosan microspheres (CM), gelatin-chitosan microspheres (GCM) and collagen-chitosan microspheres (CCM) to produce large numbers of hAMSCs for clinical trials. Growth activity measurement and differentiation essays of hAMSCs were realized. Within 2 weeks of culturing, GCMs achieved over 1.28 ± 0.06 × 10

Indexed as

Chitosan-based microspheresDual enzyme digestionEnhanced efficiencyExpansionhAMSCsIsolation

Identifiers

PMID31921407
PMCPMC6945441
OpenAlexW2999557082

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.