ArticleFrontiers in microbiology2019
Simultaneous Detection of Bovine Rotavirus, Bovine Parvovirus, and Bovine Viral Diarrhea Virus Using a Gold Nanoparticle-Assisted PCR Assay With a Dual-Priming Oligonucleotide System.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Development of a Gold Nanoparticle-Assisted Multiplex Polymerase Chain Reaction Assay for Selected Hospital-Acquired Infections-Associated Bacterial and Fungal Pathogens.ChemistryOpen · 2026Article
- Clinical applications and methodology updates in HLA typing.BMC immunology · 2026Review
- A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus, Astrovirus, and Rotavirus.Animals : an open access journal from MDPI · 2026Article
- Article
- Nanomaterials in PCR: exploring light-to-heat conversion mechanisms and microfluidic integration.Microsystems & nanoengineering · 2025Review
- Dairy Cattle Infection with Bovine Rotavirus at Different Growth Stages and Its Impact on Health and Productivity.Animals : an open access journal from MDPI · 2025Article
- Review
- Nanoparticle-assisted PCR: fundamentals, mechanisms, and forensic implications.International journal of legal medicine · 2025Review
- Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.BMC microbiology · 2025Article
- Prevalences of respiratory viruses and bacteria in Western Canadian commercial feedlot calves detected using a single metagenomic sequencing protocol vary during the first two weeks of arrival and by age group.Frontiers in veterinary science · 2025Article
- Multiplex one-step RT-qPCR assays for simultaneous detection of BRV, BCoV,Frontiers in veterinary science · 2025Article
- The Evolution of Nucleic Acid-Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metagenomic analysis reveals high diversity of gut viromes in yaks (Bos grunniens) from the Qinghai-Tibet Plateau.Communications biology · 2024Article
- Article
- Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.Frontiers in cellular and infection microbiology · 2024Article
- Simultaneous Detection of Three Subgroups of Avian Leukosis Virus Using the Nanoparticle-Assisted PCR Assay.Viruses · 2023Article
- Characterisation of the Upper Respiratory Tract Virome of Feedlot Cattle and Its Association with Bovine Respiratory Disease.Viruses · 2023Article
- A multiplex real-time fluorescence-based quantitative PCR assay for calf diarrhea viruses.Frontiers in microbiology · 2023Article
- Development of a colloidal gold immunochromatographic strip with enhanced signal for the detection of bovine parvovirus.Frontiers in microbiology · 2023Article
- Application of Nanomaterials to Enhance Polymerase Chain Reaction.Molecules (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine rotavirus (BRV), bovine parvovirus (BPV), and bovine viral diarrhea virus (BVDV) are the pathogens that cause diarrhea primarily in newborn calves. A mixed infection of BRV, BPV, and BVDV makes clinical diagnosis difficult. In this study, we designed dual-priming oligonucleotide (DPO) primers the VP6 gene of BRV, VP2 gene of BPV, and 5'UTR gene of BVDV and synthesized gold nanoparticles (GNPs) with an average diameter of 10 nm. We combined the DPOs with the GNPs to develop a DPO-nanoPCR assay for detecting BRV, BPV, and BVDV. The annealing temperature, primer concentration, and GNP concentration were optimized for this assay. Compared to a conventional PCR assay, the DPO-nanoPCR assay allowed the use of a wider range of annealing temperatures (41-65°C) to effectively amplify target genes. PCR amplification was the most efficient at 56.2°C using conventional primers. The optimal volume of all the primers (10 μM) was 1.0 μL. The optimal volume of GNPs (10 nM) for all the reactions was 0.5 μL. The detection limits of DPO-nanoPCR for pMD19-T-VP6, pMD19-T-VP2, and pMD19-T-5'UTR were 9.40 × 10
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