Evidence map›Paper›PMID 31921040›Full record

ArticleFrontiers in microbiology2019

GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target Genomes.

Ben Jia, Xueling Li, Wei Liu, Changde Lu, Xiaoting Lu, Liangxiao Ma, Yuan-Yuan Li, Chaochun Wei

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.

  1. Pooled it
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  4. Synthetic biology (Oxford, England) · 2025
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  15. Identification ofFrontiers in microbiology · 2022
    Article
  16. Development of a RT-LAMP assay for detection of SARS-CoV-2.The Indian journal of medical research · 2022
    Article
  17. Review
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ben JiaDepartment of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Xueling LiShanghai Center for Bioinformation Technology, Shanghai, China.
Wei LiuShanghai Center for Bioinformation Technology, Shanghai, China.
Changde LuShanghai Center for Bioinformation Technology, Shanghai, China.
Xiaoting LuShanghai Center for Bioinformation Technology, Shanghai, China.
Liangxiao MaShanghai Center for Bioinformation Technology, Shanghai, China.
Yuan-Yuan LiShanghai Center for Bioinformation Technology, Shanghai, China.
Chaochun WeiDepartment of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Center For Bioinformation Technology · CNShanghai Jiao Tong University · CN

Funding

Tailored Mobile Text Messaging to Reduce Problem DrinkingR34AA021502 · NIAAA · NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC · PI MUENCH, FREDERICK JOHN · 2012 to 2014
$675k
NIAAA NIH HHS R34 AA021502
6 · The paper itself

Abstract

Loop-mediated isothermal amplification (LAMP) technology has been applied in a wide range of fields such as detection of foodborne bacteria and clinical pathogens due to its simplicity and efficiency. However, existing LAMP primer designing systems require a conserved gene or a short genome region as input, and they can't design group-specific primers. With the growing number of whole genomes available, it is possible to design better primers to target a set of genomes with high specificity based on whole genomes. We present here a whole Genome based LAMP primer designer (GLAPD), a new system to design LAMP primer for a set of target genomes using whole genomes. Candidate single primer regions are identified genome wide and then combined into LAMP primer sets. For a given set of target genomes, only primer sets amplifying them and only these genomes will be output. In order to accelerate the primer designing, a GPU version is provided as well. The effectiveness of primers designed by GLAPD has been assessed for a wide range of foodborne bacteria. GLAPD can be accessed at http://cgm.sjtu.edu.cn/GLAPD/ or https://github.com/jiqingxiaoxi/GLAPD.git. A simple online version is also supplied to help users to learn and test GLAPD: http://cgm.sjtu.edu.cn/GLAPD/online/.

Indexed as

foodborne pathogensgroup-specific primerLAMPprimer designwhole genome based primer designer

Identifiers

PMID31921040
PMCPMC6923652
OpenAlexW3000699638

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.