ArticleFrontiers in microbiology2019
Impact of Sample Preservation and Manipulation on Insect Gut Microbiome Profiling. A Test Case With Fruit Flies (Diptera, Tephritidae).
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Niche Differentiation and Predicted Functions of Microbiomes in a Tri-Trophic Willow-Gall (Insects · 2025Article
- Effects of CP4-EPSPS-Containing Brassica juncea Hybrids on the Gut and Fecal Microbiota of the Terrestrial Decomposer Armadillidium vulgare.Microbial ecology · 2025Article
- Article
- Changes in life history and morphological traits over 8 generations in the brown marmorated stink bug (Hemiptera: Pentatomidae) under mass-rearing conditions.Journal of insect science (Online) · 2025Article
- Comparative gut microbiome research through the lens of ecology: theoretical considerations and best practices.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Deterministic and stochastic effects drive the gut microbial diversity in cucurbit-feeding fruit flies (Diptera, Tephritidae).PloS one · 2025Article
- Probiotic consortium modulating the gut microbiota composition and function of sterile Mediterranean fruit flies.Scientific reports · 2024Article
- Anna Karenina as a promoter of microbial diversity in the cosmopolitan agricultural pest Zeugodacus cucurbitae (Diptera, Tephritidae).PloS one · 2024Article
- Article
- Article
- "Unraveling the Gut Microbiome of the Genus Herichthys (Pisces: Cichlidae): What Can We Learn from Museum Specimens?"Current microbiology · 2022Article
- Eating eggplants as a cucurbit feeder: Dietary shifts affect the gut microbiome of the melon fly Zeugodacus cucurbitae (Diptera, Tephritidae).MicrobiologyOpen · 2022Article
- Bacterial Symbionts inInsects · 2022Article
- Evaluating Methods of Preserving Aquatic Invertebrates for Microbiome Analysis.Microorganisms · 2022Article
- AdultMicroorganisms · 2022Article
- Dynamics of the Gut Bacteriome During a Laboratory Adaptation Process of the Mediterranean Fruit Fly,Frontiers in microbiology · 2022Article
- Molecular Rationale of Insect-Microbes Symbiosis-From Insect Behaviour to Mechanism.Microorganisms · 2021Review
- Evaluation of Sample Preservation Approaches for Better Insect Microbiome Research According to Next-Generation and Third-Generation Sequencing.Microbial ecology · 2021Article
- The maintenance of microbial community in human fecal samples by a cost effective preservation buffer.Scientific reports · 2021Article
- New Insights on theMicroorganisms · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput sequencing (HTS) techniques are of great value for the investigation of microbial communities, and have been extensively used to study the gut microbiome. While most studies focus on the human gut, many others have investigated insects. However, because of the rapid spread of HTS techniques, a lot of variation exists in the protocols for sample preparation. In the present study, we investigated the impact of two widely adopted sample-processing procedures preceding library preparation, i.e., preservation of insect tissue in 70% ethanol (EtOH) and sample dissection. We used the fruit fly
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.