ArticleFrontiers in cellular neuroscience2019
Genetic Modification of Brain Organoids.
Article in Frontiers in cellular neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Using Brain Organoids to Explore Human Neurobiology.Cellular and molecular neurobiology · 2026Review
- Metabolic interactions in the brain: the crucial roles of neurons, astrocytes, and microglia in health and disease.Frontiers in neuroscience · 2026Review
- Modeling Alzheimer's disease with brain organoids: mechanisms, applications, and future directions.Frontiers in cell and developmental biology · 2026Review
- Generating cerebellar organoids from pluripotent stem cells.Disease models & mechanisms · 2026Review
- Advances in organoids for personalized medicine: from technological development to clinical application.Frontiers in cell and developmental biology · 2026Review
- A neurorecording toolkit for longitudinal assessments of transplanted human cortical organoidsbioRxiv : the preprint server for biology · 2025Article
- Advances in Human Brain Organoids: Methodological Innovations and Future Directions for Drug Discovery.Current drug research reviews · 2025Review
- Multiscale engineering of brain organoids for disease modeling.Advanced drug delivery reviews · 2024Review
- Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics.Biotechnology and bioengineering · 2024Review
- Advanced Cellular Models for Rare Disease Study: Exploring Neural, Muscle and Skeletal Organoids.International journal of molecular sciences · 2024Review
- Modeling glioblastoma complexity with organoids for personalized treatments.Trends in molecular medicine · 2023Review
- Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification.Journal of visualized experiments : JoVE · 2023Article
- Review
- Human Brain Organoids in Migraine Research: Pathogenesis and Drug Development.International journal of molecular sciences · 2023Article
- The SVZ stem cell niche-components, functions, andFrontiers in cell and developmental biology · 2023Review
- Human-specific ARHGAP11B ensures human-like basal progenitor levels in hominid cerebral organoids.EMBO reports · 2022Article
- A next-generation iPSC-derived forebrain organoid model of tauopathy with tau fibrils by AAV-mediated gene transfer.Cell reports methods · 2022Article
- Injection and electroporation of plasmid DNA into human cortical organoids.STAR protocols · 2022Article
- The Future of 3D Brain Cultures in Developmental Neurotoxicity Testing.Frontiers in toxicology · 2022Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brain organoids have become increasingly used systems allowing 3D-modeling of human brain development, evolution, and disease. To be able to make full use of these modeling systems, researchers have developed a growing toolkit of genetic modification techniques. These techniques can be applied to mature brain organoids or to the preceding embryoid bodies (EBs) and founding cells. This review will describe techniques used for transient and stable genetic modification of brain organoids and discuss their current use and respective advantages and disadvantages. Transient approaches include adeno-associated virus (AAV) and electroporation-based techniques, whereas stable genetic modification approaches make use of lentivirus (including viral stamping), transposon and CRISPR/Cas9 systems. Finally, an outlook as to likely future developments and applications regarding genetic modifications of brain organoids will be presented.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.