ArticleScientific reports2020
Rapid and cost-effective generation of single specimen multilocus barcoding data from whole arthropod communities by multiple levels of multiplexing.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
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- Syrphid Diversity in Sweet Alyssum Flower Strips in Quebec's Lettuce Fields: Molecular Identification and Delimitation of theEcology and evolution · 2025Article
- Insights from draft genomes of Heterodera species isolated from field soil samples.BMC genomics · 2025Article
- Three steps towards comparability and standardization among molecular methods for characterizing insect communities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Article
- Toward global integration of biodiversity big data: a harmonized metabarcode data generation module for terrestrial arthropods.GigaScience · 2022Article
- The bug in a teacup-monitoring arthropod-plant associations with environmental DNA from dried plant material.Biology letters · 2022Article
- Method for quick DNA barcode reference library construction.Ecology and evolution · 2021Article
- DNA barcodes enable higher taxonomic assignments in the Acari.Scientific reports · 2021Article
- Metabarcoding Malaise traps and soil eDNA reveals seasonal and local arthropod diversity shifts.Scientific reports · 2021Article
- Optimization and validation of a cost-effective protocol for biosurveillance of invasive alien species.Ecology and evolution · 2021Article
- DNA barcodes of birds from northern Colombia.Biodiversity data journal · 2021Article
- High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities.Development genes and evolution · 2020Review
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In light of the current biodiversity crisis, molecular barcoding has developed into an irreplaceable tool. Barcoding has been considerably simplified by developments in high throughput sequencing technology, but still can be prohibitively expensive and laborious when community samples of thousands of specimens need to be processed. Here, we outline an Illumina amplicon sequencing approach to generate multilocus data from large collections of arthropods. We reduce cost and effort up to 50-fold, by combining multiplex PCRs and DNA extractions from pools of presorted and morphotyped specimens and using two levels of sample indexing. We test our protocol by generating a comprehensive, community wide dataset of barcode sequences for several thousand Hawaiian arthropods from 14 orders, which were collected across the archipelago using various trapping methods. We explore patterns of diversity across the Archipelago and compare the utility of different arthropod trapping methods for biodiversity explorations on Hawaii, highlighting undergrowth beating as highly efficient method. Moreover, we show the effects of barcode marker, taxonomy and relative biomass of the targeted specimens and sequencing coverage on taxon recovery. Our protocol enables rapid and inexpensive explorations of diversity patterns and the generation of multilocus barcode reference libraries across whole ecosystems.
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