ArticleMalaria journal2020
Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma.
Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Parasitaemia and fever in uncomplicated Plasmodium vivax malaria: A systematic review and individual patient data meta-analysis.PLoS neglected tropical diseases · 2025Pooled it
- Evaluation of CareStart™ malaria HRP2/pLDH (Pf/PAN) combo rapid diagnostic test for diagnosis of Plasmodium falciparum infection in malaria co-endemic areas in association with parasite density.Malaria journal · 2025Trial
- Persistence of Plasmodium falciparum HRP-2 antigenaemia after artemisinin combination therapy is not associated with gametocytes.Malaria journal · 2022Trial
- Ultrasensitive ELISA for Accurate Detection ofOpen forum infectious diseases · 2025Article
- Evaluating the performance of an artificial intelligence-based electronic reader for malaria rapid diagnostic tests across Benin, Côte d'Ivoire, Nigeria and Uganda.Malaria journal · 2025Article
- CD8Frontiers in cellular and infection microbiology · 2025Article
- Use of latex microbeads for detection of Plasmodium vivax lactate dehydrogenase using flow cytometry.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Article
- Comparative evaluation of the diagnostic accuracies of four different malaria rapid diagnostic test kits available in Ghana.PloS one · 2024Article
- Malaria in pregnancy (MiP) studies assessing the clinical performance of highly sensitive rapid diagnostic tests (HS-RDT) for Plasmodium falciparum detection.Malaria journal · 2023Review
- Factors Affecting the Performance of HRP2-Based Malaria Rapid Diagnostic Tests.Tropical medicine and infectious disease · 2022Review
- Magnetic Bead Handling Using a Paper-Based Device for Quantitative Point-of-Care Testing.Biosensors · 2022Article
- Field performance of Plasmodium falciparum lactate dehydrogenase rapid diagnostic tests during a large histidine-rich protein 2 deletion survey in Ethiopia.Malaria journal · 2022Article
- Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens.Malaria journal · 2022Article
- Performance and utility of more highly sensitive malaria rapid diagnostic tests.BMC infectious diseases · 2022Article
- Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018.Scientific reports · 2021Article
- PfHRP2 detection using plasmonic optrodes: performance analysis.Malaria journal · 2021Article
- Laboratory Detection of Malaria Antigens: a Strong Tool for Malaria Research, Diagnosis, and Epidemiology.Clinical microbiology reviews · 2021Article
- Assessment ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Field performance of ultrasensitive and conventional malaria rapid diagnostic tests in southern Mozambique.Malaria journal · 2020Article
- Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood.Nature communications · 2020Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in different endemic countries.
resultsThe qSAT was specific for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r = 0.59 and 0.75, respectively) as well as microscopy (Spearman r = 0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density.
conclusionThis immunoassay can be used as a reference test for the detection and quantification of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings.
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