Evidence map›Paper›PMID 31916138›Full record

ArticleVirus genes2020

Screening pigs for xenotransplantation in China: investigation of porcine endogenous retrovirus in Diannan small-eared pigs.

Yunfei Zhang, Xiaowei Xing, Lihua Huang, Yong Wu, Peng Li, Ruhong Li, Gang Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yunfei ZhangCenter for Medical Experiments, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Xiaowei XingCenter for Medical Experiments, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Lihua HuangCenter for Medical Experiments, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Yong WuClinical Laboratory, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Peng LiDepartment of General Surgery, Yanan Hospital Affiliated to Kunming Medical University, Kunming, 650051, Yunnan, China.
Ruhong LiDepartment of General Surgery, Yanan Hospital Affiliated to Kunming Medical University, Kunming, 650051, Yunnan, China. lrh272@126.com.
Gang LiuThe Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, 410078, Hunan, China. liugang7378@sina.com.
Central South University · CNKunming Medical University · CNThird Xiangya Hospital · CN

Funding

Major Research Plan 81560140
6 · The paper itself

Abstract

Porcine endogenous retrovirus (PERV), which integrates as a provirus into the genome of pig cells, is an important biosafety issue in xenotransplantation. Screening and analyzing the presence and expression of PERV will provide essential parameters for assessing the biosafety of donor sources. In the present study, we investigated the prevalence of PERV in Diannan small-eared pigs, a unique closed colony that is distributed in southern Yunnan Province in southwestern China. PCR was performed to amplify env-A, env-B, env-C, pol, gag, and mtDNA in peripheral blood samples. The results revealed that PERV env-A, env-B, pol, and gag were detected in all individuals, but env-C was deficient in most pigs, suggesting that the main subtypes of PERVs in Diannan small-eared pigs are PERV-A and PERV-B. Furthermore, PERV pol and the porcine housekeeping gene GAPDH were detected by RT-PCR in all peripheral blood samples, indicating that PERV had transcriptional activity. Finally, the consensus sequences of PERV-A and PERV-B were amplified and digested with KpnI and MboI. Interestingly, a total of seven digestion patterns were obtained, which is less than that observed in other pig breeds. The PCR products were cloned into the pUCm-T vector and sequenced. The results showed that all of the inserts were highly homologous to either PERV-A or PERV-B, and the ratios of PERV-A and PERV-B were 21.1% and 78.9%, respectively. These data suggest that Diannan small-eared pigs may be a candidate donor source for xenotransplantation.

Indexed as

Transplantation, HeterologousAnimalsChinaEndogenous RetrovirusesRNA, ViralSwineRNA, ViralDiannan small-eared pigDonorPERVSubtypeXenotransplantation

Identifiers

PMID31916138
OpenAlexW2999328122

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.