ArticleCirculation research2020
Three-Dimensional Imaging Provides Detailed Atherosclerotic Plaque Morphology and Reveals Angiogenesis After Carotid Artery Ligation.
Article in Circulation research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Characterization of carotid remodeling in gestational hypertension using radiofrequency data-based vessel stiffness analysis and vector flow imaging.Quantitative imaging in medicine and surgery · 2026Article
- Hemi-ellipsoid formula enables accurate assessment of carotid plaque volume and atherosclerotic burden.Scientific reports · 2026Article
- SCAP Interacts with Trim27 to Promote Vascular Neointimal Hyperplasia by Regulating the Stability and Ubiquitination of IκBα.Aging and disease · 2025Article
- Advanced Imaging Techniques for Atherosclerosis and Cardiovascular Calcification in Animal Models.Journal of cardiovascular development and disease · 2024Review
- Advances in secondary prevention mechanisms of macrovascular complications in type 2 diabetes mellitus patients: a comprehensive review.European journal of medical research · 2024Review
- Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis.Heliyon · 2023Article
- Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.Journal of biophotonics · 2023Article
- A dataset of rodent cerebrovasculature from in vivo multiphoton fluorescence microscopy imaging.Scientific data · 2023Article
- Characteristics and evaluation of atherosclerotic plaques: an overview of state-of-the-art techniques.Frontiers in neurology · 2023Review
- Tissue Clearing Approaches in Atherosclerosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Three-Dimensional Visualization of Atherosclerotic Vessels by Tissue Clearing and Light-Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- 2D Projection Maps of WSS and OSI Reveal Distinct Spatiotemporal Changes in Hemodynamics in the Murine Aorta during Ageing and Atherosclerosis.Biomedicines · 2021Article
- Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography.Scientific reports · 2021Article
- The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries.Stem cell research & therapy · 2021Article
- Light sheet fluorescence microscopy for quantitative three-dimensional imaging of vascular remodelling.Cardiovascular research · 2021Article
- Sodium Selenite Attenuates Balloon Injury-Induced and Monocrotaline-Induced Vascular Remodeling in Rats.Frontiers in pharmacology · 2021Article
- Regenerated Microvascular Networks in Ischemic Skeletal Muscle.Frontiers in physiology · 2021Article
- Diagnosis and treatment of carotid body tumors.American journal of translational research · 2021Article
- Deep learning reveals 3D atherosclerotic plaque distribution and composition.Scientific reports · 2020Article
- A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application.Journal of visualized experiments : JoVE · 2020Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
rationaleRemodeling of the vessel wall and the formation of vascular networks are dynamic processes that occur during mammalian embryonic development and in adulthood. Plaque development and excessive neointima formation are hallmarks of atherosclerosis and vascular injury. As our understanding of these complex processes evolves, there is a need to develop new imaging techniques to study underlying mechanisms.
objectiveWe used tissue clearing and light-sheet microscopy for 3-dimensional (3D) profiling of the vascular response to carotid artery ligation and induction of atherosclerosis in mouse models. METHODS AND
resultsAdipo-Clear and immunolabeling in combination with light-sheet microscopy were applied to image carotid arteries and brachiocephalic arteries, allowing for 3D reconstruction of vessel architecture. Entire 3D neointima formations with different geometries were observed within the carotid artery and scored by volumetric analysis. Additionally, we identified a CD31-positive adventitial plexus after ligation of the carotid artery that evolved and matured over time. We also used this method to characterize plaque extent and composition in the brachiocephalic arteries of ApoE-deficient mice on high-fat diet. The plaques exhibited inter-animal differences in terms of plaque volume, geometry, and ratio of acellular core to plaque volume. A 3D reconstruction of the endothelium overlying the plaque was also generated.
conclusionsWe present a novel approach to characterize vascular remodeling in adult mice using Adipo-Clear in combination with light-sheet microscopy. Our method reconstructs 3D neointima formation after arterial injury and allows for volumetric analysis of remodeling, in addition to revealing angiogenesis and maturation of a plexus surrounding the carotid artery. This method generates complete 3D reconstructions of atherosclerotic plaques and uncovers their volume, geometry, acellular component, surface, and spatial position within the brachiocephalic arteries. Our approach may be used in a number of mouse models of cardiovascular disease to assess vessel geometry and volume. Visual Overview: An online visual overview is available for this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.