Evidence map›Paper›PMID 31913687›Full record

Observational studyAmerican journal of physiology. Regulatory, integrative and comparative physiology2020

Cell-free mitochondrial DNA increases in maternal circulation during healthy pregnancy: a prospective, longitudinal study.

Spencer C Cushen, Marc L Sprouse, Alexandra Blessing, Jie Sun, Sara S Jarvis, Yoshiyuki Okada, Qi Fu, Steven A Romero, Nicole R Phillips, Styliani Goulopoulou

Open access · bronzeAbstract readComparative StudyObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in American journal of physiology. Regulatory, integrative and comparative physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Observational
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Mitochondrial signal transduction.Cell metabolism · 2022
    Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Spencer C CushenDepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas.
Marc L SprouseDepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas.
Alexandra BlessingDepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas.
Jie SunDepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas.
Sara S JarvisDivision of Cardiology, Internal Medicine, University of Texas Southwestern Medical Center, Institute for Exercise and Environmental Medicine, Texas Health Presbyterian Hospital Dallas, Dallas, Texas.
Yoshiyuki OkadaDivision of Cardiology, Internal Medicine, University of Texas Southwestern Medical Center, Institute for Exercise and Environmental Medicine, Texas Health Presbyterian Hospital Dallas, Dallas, Texas.
Qi FuDivision of Cardiology, Internal Medicine, University of Texas Southwestern Medical Center, Institute for Exercise and Environmental Medicine, Texas Health Presbyterian Hospital Dallas, Dallas, Texas.
Steven A RomeroDepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas.
Nicole R PhillipsDepartment of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas.
Styliani GoulopoulouDepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas.ORCID 0000-0001-7636-8256
University of North Texas · USTexas Health Dallas · US

Funding

Vasomotor Sympathetic Activity during Early Pregnancy in HumansR21HL088184 · NHLBI · UT SOUTHWESTERN MEDICAL CENTER · PI FU, QI · 2009 to 2010
$424k
NHLBI NIH HHS R21 HL088184
6 · The paper itself

Abstract

Mitochondrial DNA (mtDNA) exposed to the extracellular space due to cell death has immunostimulatory properties. Case-control studies reported a positive association between odds of developing preeclampsia and circulating mtDNA. These findings are based on relative quantification protocols that do not allow determination of absolute concentrations of mtDNA and are highly sensitive to nuclear DNA contamination. Furthermore, circulating mtDNA concentrations in response to normal pregnancy, which is an inflammatory state characterized by continuous placental cell apoptosis, have not been established. The main objective of this study was to determine longitudinal changes in circulating mtDNA from preconception to first trimester, third trimester, and postpartum in healthy pregnant women. Absolute real-time PCR quantification of mtDNA and nuclear DNA (nDNA) was performed on whole genomic extracts from serum using TaqMan probes and chemistry. Serum cell-free mtDNA and nDNA concentrations were greater in late pregnancy as compared with early pregnancy and postpartum. Pregnant women carrying neonates at the upper quartile of birth length distribution had higher concentrations of mtDNA in late pregnancy compared with pregnancies carrying neonates at the lower quartile. The correlation between circulating mtDNA and nDNA concentrations varied by sex (i.e., pregnancies carrying female vs. male fetuses). This study is the first to establish temporal patterns of circulating cell-free mtDNA concentrations in normal human pregnancy using absolute DNA quantification techniques. Concentrations of circulating mtDNA in normal pregnancy may be used as reference values for the development of clinical prognostic or diagnostic tests in pregnant women with, or at risk of developing, gestational complications.

Indexed as

AdultCell-Free Nucleic AcidsDNA, MitochondrialFemaleGenetic MarkersHealthy VolunteersHumansLongitudinal StudiesPostpartum PeriodPregnancyPregnancy TrimestersProspective StudiesSex CharacteristicsSex Determination ProcessesYoung AdultCell-Free Nucleic AcidsDNA, MitochondrialGenetic Markerscell-free DNADNA quantificationgestational agemitochondria

Identifiers

PMID31913687
PMCPMC7052592
OpenAlexW3000264360

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.