Evidence map›Paper›PMID 31913120›Full record

ArticleeLife2020

Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas.

Alison B Hickman, Shweta Kailasan, Pavol Genzor, Astrid D Haase, Fred Dyda

Open access · goldAbstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 21 citations in OpenAlex.

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  10. Label-free methods for opticalPhysical chemistry chemical physics : PCCP · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Alison B HickmanLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.ORCID 0000-0001-7666-0249
Shweta KailasanLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.ORCID 0000-0003-0876-6812
Pavol GenzorLaboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.
Astrid D HaaseLaboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.
Fred DydaLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.ORCID 0000-0003-1689-9041
National Institutes of Health · US

Funding

PIWI proteins and PIWI-interacting small RNAs guard the genomic integrity of germ cellsZIADK075111 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI HAASE, ASTRID · 2015 to 2025
$14.7M
Mechanism of casposon mediated genome rearrangementsZIADK075131 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI DYDA, FREDERICK · 2016 to 2020
$3.9M
Phase II SER-CAT Optimization: Acquisition of a next generation area detectorS10RR028976 · NCRR · UNIVERSITY OF GEORGIA · PI WANG, BI-CHENG · 2010 to 2010
$1.5M
NCRR NIH HHS S10 RR028976
6 · The paper itself

Abstract

Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. It is hypothesized that Cas1 evolved from casposases, a novel class of transposases. We show here that the

Indexed as

CRISPR-Cas SystemsArchaeal ProteinsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated ProteinsDNADNA, ArchaealDNA, IntergenicDNA Transposable ElementsMethanosarcinaModels, MolecularNucleic Acid ConformationProtein ConformationProtein MultimerizationTransposasesArchaeal ProteinsCRISPR-Associated ProteinsDNADNA, ArchaealDNA, IntergenicDNA Transposable ElementsTransposasesCas1casposonCRISPR-Casintegrasemobile genetic elementmolecular biophysicsstructural biologytransposon

Identifiers

PMID31913120
PMCPMC6977970
OpenAlexW2998897949

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.