Evidence map›Paper›PMID 31911759›Full record

ArticleJournal of inflammation (London, England)2020

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia.

Mu Xu, Yi Yan, Mengye Zhu, Zhijian Wang, Xuexue Zhang, Daying Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of inflammation (London, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mu XuDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
Yi YanDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
Mengye ZhuDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
Zhijian WangDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
Xuexue ZhangDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
Daying ZhangDepartment of Pain Medicine, The first affiliated hospital of NanChang university, 17 Yongwai Zhengjie, Donghu District, Nanchang, 330006 Jiangxi China.
First Affiliated Hospital of Nanchang University · CNNanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThis study aims to investigate the role of long non-coding RNA Gm14461 in regulating pain transmission in trigeminal neuralgia (TN). The mouse TN model was produced by chronic constriction injury of the infraorbital nerve (CCI-ION). The values of mechanical withdrawal threshold (MWT) were measured to assess the nociception of mice at different times after CCI-ION surgery (0, 1, 3, 5, 7, 9, 11, 13, 15 d). The primary mouse trigeminal ganglion neurons (TGNs) were isolated from C57BL/6 J mice and treated with TNF-α to mimic a TN cellular model. The expression of Gm14461, TNF-α, IL-1β, and IL-6 was examined using qRT-PCR. The protein levels of CGRP and P2X3/7 receptor were measured using western blot.

resultsGm14461 expression was increased in trigeminal ganglia (TGs) of TN mice on the operation side. Furthermore, Gm14461 knockdown in TGs increased, whereas Gm14461 overexpression decreased MWT in TN mice. Moreover, Gm14461 knockdown downregulated, whereas Gm14461 overexpression upregulated mRNA levels of TNF-α, IL-1β, and IL-6 and protein levels of CGRP and P2X3/7 receptor in TGs from TN mice. In vitro assay showed that Gm14461 was upregulated by TNF-α, IL-1β, and IL-6. Additionally, Gm14461 knockdown decreased protein levels of CGRP and P2X3/7 receptor in TNF-α-treated TGNs, whereas Gm14461 overexpression exerted the opposite effect.

conclusionGm14461 promoted pain transmission (reduced MWT value) in a CCI-ION-induced mouse TN model. The underlying mechanisms might involve the regulation of pro-inflammatory cytokines, CGRP and P2X3/7 receptor.

Indexed as

CGRPGm14461InflammationP2X3 receptorP2X7 receptorTrigeminal neuralgia

Identifiers

PMID31911759
PMCPMC6942393
OpenAlexW3010964004

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.