ArticleNature communications2020
Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed.
- Zinc-Doping Trap Engineering and FRET Synergy Enable Multicolor Room-Temperature Phosphorescence in Rice-Derived Carbon Dots Confined in Amorphous Alumina for Advanced Encryption.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts.Communications chemistry · 2025Article
- Charge engineering controls cooperative assembly and loading in protein host-guest complexes.Journal of materials chemistry. B · 2025Article
- HP1 loses its chromatin clustering and phase separation function across evolution.Nature communications · 2025Article
- Single-shot two-dimensional nano-size mapping of fluorescent molecules by ultrafast polarization anisotropy imaging.Nature communications · 2025Article
- Article
- From Monomers to Hexamers: A Theoretical Probability of the Neighbor Density Approach to Dissect Protein Oligomerization in Cells.Analytical chemistry · 2024Article
- Quantitative fluorescence emission anisotropy microscopy for implementing homo-fluorescence resonance energy transfer measurements in living cells.Molecular biology of the cell · 2023Article
- Sequential Two-Photon Delayed Fluorescence Anisotropy for Macromolecular Size Determination.The journal of physical chemistry. B · 2023Article
- Imaging the rotational mobility of carbon dot-gold nanoparticle conjugates using frequency domain wide-field time-resolved fluorescence anisotropy.Journal of biomedical optics · 2023Article
- Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence Anisotropy.Chemical & biomedical imaging · 2023Article
- Extending fluorescence anisotropy to large complexes using reversibly switchable proteins.Nature biotechnology · 2023Article
- Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification.Nature communications · 2022Article
- Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein.Micromachines · 2021Article
- Simultaneous readout of multiple FRET pairs using photochromism.Nature communications · 2021Article
- POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry.Current opinion in structural biology · 2021Review
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Monitoring of protein oligomerization has benefited greatly from Förster Resonance Energy Transfer (FRET) measurements. Although donors and acceptors are typically fluorescent molecules with different spectra, homo-FRET can occur between fluorescent molecules of the same type if the emission spectrum overlaps with the absorption spectrum. Here, we describe homo-FRET measurements by monitoring anisotropy changes in photoswitchable fluorescent proteins while photoswitching to the off state. These offer the capability to estimate anisotropy in the same specimen during homo-FRET as well as non-FRET conditions. We demonstrate photoswitching anisotropy FRET (psAFRET) with a number of test chimeras and example oligomeric complexes inside living cells. We also present an equation derived from FRET and anisotropy equations which converts anisotropy changes into a factor we call delta r FRET (drFRET). This is analogous to an energy transfer efficiency and allows experiments performed on a given homo-FRET pair to be more easily compared across different optical configurations.
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